Novel magnetic nanoparticle-containing adhesive with greater dentin bond strength and antibacterial and remineralizing capabilities.
Novel magnetic nanoparticle-containing adhesive with greater dentin bond strength and antibacterial and remineralizing capabilities.
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DOI：
10.1016/j.dental.2018.06.001
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发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Xu HHK
中科院分区：
文献类型：
--
作者：
Li Y;Hu X;Xia Y;Ji Y;Ruan J;Weir MD;Lin X;Nie Z;Gu N;Masri R;Chang X;Xu HHK
最近开发了一种可以用磁力控制的纳米颗粒掺杂粘合剂，可将药物输送到牙髓并改善粘合剂对牙本质的渗透。然而，它不具有杀菌和再矿化能力。本研究的目的是：（1）开发一种含有甲基丙烯酸二甲氨基十六烷基酯（DMAHDM）、无定形磷酸钙纳米粒子（NACP）和磁性纳米粒子（MNP）的磁性纳米粒子粘合剂； (2) 研究对牙本质粘合强度、钙 (Ca) 和磷酸 (P) 离子释放以及抗生物膜特性的影响。 MNP、DMAHDM 和 NACP 分别以质量比 2%、5% 和 20% 混合到 Scotchbond SBMP 中。使用两种类型的磁性纳米颗粒：丙烯酸酯功能化铁纳米颗粒（AINP）；和氧化铁纳米颗粒（IONP）。每种类型均以1质量%添加到树脂中。牙本质粘合通过使用市售立方体磁铁提供的磁力施加 3 分钟来进行。测量牙本质剪切粘合强度。变形链球菌生物膜在树脂上生长，并测定代谢活性、乳酸和菌落形成单位 (CFU)。测量了生物膜培养基中的 Ca 和 P 离子浓度以及 pH 值。使用磁力的含磁性纳米粒子的粘合剂将牙本质剪切粘合强度比 SBMP 对照提高了 59% (p < 0.05)。添加 DMAHDM 和 NACP 不会对牙本质粘合强度产生不利影响 (p > 0.05)。含有 MNP+DMAHDM+NACP 的粘合剂将变形链球菌生物膜 CFU 降低了 4 个对数。对于具有 NACP 的粘合剂，生物膜介质成为 Ca 和 P 离子储存库。含有NACP的磁性纳米粒子粘合剂的生物膜培养基的安全pH值为6.9，而商业粘合剂的生物膜培养基的致龋pH值为4.5。含有磁性纳米颗粒的粘合剂与 DMAHDM 和 NACP 在磁力作用下产生的牙本质粘合强度比商业控制要高得多。这种新型粘合剂将生物膜 CFU 降低了 4 个对数级，并将生物膜 pH 值从致龋 pH 值 4.5 提高到 6.9，因此有望增强树脂与牙齿的粘合，强化牙齿结构，并抑制修复体边缘的继发龋。
A nanoparticle-doped adhesive that can be controlled with magnetic forces was recently developed to deliver drugs to the pulp and improve adhesive penetration into dentin. However, it did not have bactericidal and remineralization abilities. The objectives of this study were to: (1) develop a magnetic nanoparticle-containing adhesive with dimethylaminohexadecyl methacrylate (DMAHDM), amorphous calcium phosphate nanoparticles (NACP) and magnetic nanoparticles (MNP); and (2) investigate the effects on dentin bond strength, calcium (Ca) and phosphate (P) ion release and anti-biofilm properties. MNP, DMAHDM and NACP were mixed into Scotchbond SBMP at 2%, 5% and 20% by mass, respectively. Two types of magnetic nanoparticles were used: acrylate-functionalized iron nanoparticles (AINPs); and iron oxide nanoparticles (IONPs). Each type was added into the resin at 1% by mass. Dentin bonding was performed with a magnetic force application for 3 minutes, provided by a commercial cube-shaped magnet. Dentin shear bond strengths were measured. Streptococcus mutans biofilms were grown on resins, and metabolic activity, lactic acid and colony-forming units (CFU) were determined. Ca and P ion concentrations in, and pH of biofilm culture medium were measured. Magnetic nanoparticle-containing adhesive using magnetic force increased the dentin shear bond strength by 59% over SBMP Control (p < 0.05). Adding DMAHDM and NACP did not adversely affect the dentin bond strength (p > 0.05). The adhesive with MNP+DMAHDM+NACP reduced the Streptococcus mutans biofilm CFU by 4 logs. For the adhesive with NACP, the biofilm medium became a Ca and P ion reservoir. The biofilm culture medium of the magnetic nanoparticle-containing adhesive with NACP had a safe pH of 6.9, while the biofilm medium of commercial adhesive had a cariogenic pH of 4.5. Magnetic nanoparticle-containing adhesive with DMAHDM and NACP under a magnetic force yielded much greater dentin bond strength than commercial control. The novel adhesive reduced biofilm CFU by 4 logs and increased the biofilm pH from a cariogenic pH 4.5 to 6.9, and therefore is promising to enhance the resin-tooth bond, strengthen tooth structures, and suppress secondary caries at the restoration margins.
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影响因子：
4.6
作者：
Huang L;Yu F;Sun X;Dong Y;Lin PT;Yu HH;Xiao YH;Chai ZG;Xing XD;Chen JH
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影响因子：
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作者：
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影响因子：
3
作者：
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通讯作者：
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影响因子：
5
作者：
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通讯作者：
Ferracane, Jack L.
DOI：
10.5301/jabfm.5000337
发表时间：
2017-04-01
影响因子：
2.5
作者：
Farrugia, Cher;Haider, Julie;Camilleri, Josette
通讯作者：
Camilleri, Josette