Screening active components from Yu-ping-feng-san for regulating initiative key factors in allergic sensitization.
Screening active components from Yu-ping-feng-san for regulating initiative key factors in allergic sensitization.
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筛选玉屏风散调控过敏致敏主动关键因素的活性成分
DOI：
10.1371/journal.pone.0107279
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hong M
中科院分区：
文献类型：
--
作者：
Shen D;Xie X;Zhu Z;Yu X;Liu H;Wang H;Fan H;Wang D;Jiang G;Hong M
玉屏风散(YPFS)是临床上用于治疗过敏性疾病的一种中药配方，具有减少过敏复发的特点。我们以前的研究表明，YPFS有效地抑制了变应性炎症中的T辅助细胞因子2。YPF及其有效成分的基本作用机制尚不清楚。在这项研究中，研究表明，YPFS在体外和体内都显著抑制胸腺基质淋巴生成素(TSLP)的产生，TSLP是变态反应性炎症中的一种上皮细胞衍生的主动因子。建立了16HBE与人支气管上皮细胞(16HBE)结合的高效液相色谱质谱(16HBE-HPLCMS)联用方法，以探索其潜在的活性成分。与16HBE细胞结合的五种成分为：毛蕊异黄素-7-葡萄糖苷、软骨素、粘菌素、β-葡萄糖基胡芦醇和芒柄花素。对YPFS处理小鼠的血清进行了分析，检测出粘附素、芒柄花素和西米夫净三种主要成分。其中，粘附素和芒柄花素在16HBE-HPLC-MS和YPFS处理的小鼠血清中均有检测到。在体内变态反应性炎症初期，克雷考星和芒柄花素能显著降低TSLP水平。粘附素和芒柄花素在体外也能抑制核转录因子κB的转录激活或核转位。在特应性接触性皮炎小鼠模型中，仅在初始阶段给予粘附素和芒柄花素可减轻过敏性炎症。因此，上皮细胞结合高效液相色谱-质谱法是从复杂中药混合物中筛选有效成分的有效方法。结果表明，从YPFS中筛选出的化合物可显着减轻变态反应性炎症，其机制可能是通过调节NF-κB的激活来减少TSLP的产生。
Yu-ping-feng-san (YPFS) is a Chinese medical formula that is used clinically for allergic diseases and characterized by reducing allergy relapse. Our previous studies demonstrated that YPFS efficiently inhibited T helper 2 cytokines in allergic inflammation. The underlying mechanisms of action of YPFS and its effective components remain unclear. In this study, it was shown that YPFS significantly inhibited production of thymic stromal lymphopoietin (TSLP), an epithelial cell-derived initiative factor in allergic inflammation, in vitro and in vivo. A method of human bronchial epithelial cell (16HBE) binding combined with HPLC-MS (named 16HBE-HPLC-MS) was established to explore potential active components of YPFS. The following five components bound to 16HBE cells: calycosin-7-glucoside, ononin, claycosin, sec-o-glucosylhamaudol and formononetin. Serum from YPFS-treated mice was analyzed and three major components were detected claycosin, formononetin and cimifugin. Among these, claycosin and formononetin were detected by 16HBE-HPLC-MS and in the serum of YPFS-treated mice. Claycosin and formononetin decreased the level of TSLP markedly at the initial stage of allergic inflammation in vivo. Nuclear factor (NF)-κB, a key transcription factor in TSLP production, was also inhibited by claycosin and formononetin, either in terms of transcriptional activation or its nuclear translocation in vitro. Allergic inflammation was reduced by claycosin and formononetin when they are administered only at the initial stage in a murine model of atopic contact dermatitis. Thus, epithelial cell binding combined with HPLC-MS is a valid method for screening active components from complex mixtures of Chinese medicine. It was demonstrated that the compounds screened from YPFS significantly attenuated allergic inflammation probably by reducing TSLP production via regulating NF-κB activation.
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DOI：
10.1084/jem.20062211
发表时间：
2007-02-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Allakhverdi Z;Comeau MR;Jessup HK;Yoon BR;Brewer A;Chartier S;Paquette N;Ziegler SF;Sarfati M;Delespesse G
通讯作者：
Delespesse G
建立了一种与HPLC结合的肝细胞提取方法，用于筛选中药中潜在的活性成分 - 将赫巴尔·阿耳米西亚·斯科帕里亚（Herba Artemisiae scopariae）作为一种例证方法。
DOI：
10.3390/molecules17021468
发表时间：
2012-02-06
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Hong M;Ma HY;Wu XR;Hua YQ;Zhu Q;Fan HW
通讯作者：
Fan HW
影响因子：
8.7
作者：
Saenz SA;Taylor BC;Artis D
通讯作者：
Artis D
DOI：
10.1046/j.1540-8167.2005.40584.x
发表时间：
2005-06-01
影响因子：
2.7
作者：
Brigadeau, F;Gelé, P;Lacroix, D
通讯作者：
Lacroix, D
DOI：
10.1084/jem.20051745
发表时间：
2006-02-20
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Liu YJ
通讯作者：
Liu YJ