A method of hepatocyte extraction conjugated with HPLC is established for screening potential active components in Chinese medicines--probing Herba Artemisiae Scopariae as an exemplifying approach.
A method of hepatocyte extraction conjugated with HPLC is established for screening potential active components in Chinese medicines--probing Herba Artemisiae Scopariae as an exemplifying approach.
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建立了一种与HPLC结合的肝细胞提取方法，用于筛选中药中潜在的活性成分 - 将赫巴尔·阿耳米西亚·斯科帕里亚（Herba Artemisiae scopariae）作为一种例证方法。
DOI：
10.3390/molecules17021468
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发表时间：
2012-02-06
期刊：
影响因子：
--
通讯作者：
Fan HW
中科院分区：
文献类型：
--
作者：
Hong M;Ma HY;Wu XR;Hua YQ;Zhu Q;Fan HW
为了建立一种有效、快速的筛选中药中潜在生物活性化合物的方法，本研究采用肝细胞法提取治疗肝脏疾病的临床药物联苯酯或治疗由病毒、化学物质或酒精引起的肝炎等肝脏疾病的中药复方蒿属（a . Scopariae）中的化学物质。肝细胞提取后，用高效液相色谱法对化合物进行分析，因此这种方法被称为肝细胞提取共轭高效液相色谱法（HE-HPLC）。在本研究的第一部分，HE-HPLC显示，联苯酯是由肝细胞提取的，并通过HPLC-DAD检测，表明了该方法的可行性。然后在研究的第二部分，采用HE-HPLC法研究了黄芪提取物中潜在的有效成分。用HPLC-DAD法检测了黄连提取物中6种能与肝细胞结合的化学成分，鉴定出3种分别为7-羟基香豆素（7-OH-C）、毛细蒿素A和7-甲氧基香豆素。体外实验表明，7-OH-C可保护HL-7702肝细胞免受H2O2损伤。结果表明，这些化合物可被肝细胞提取，高效液相色谱法检测，更重要的是具有生物活性。提示HE-HPLC是一种有效筛选肝类中药有效成分的有效方法。
In order to establish an effective and quick method for screening potential bioactive compounds in Traditional Chinese Medicines (TCMs), hepatocytes were employed for extracting either bifendate, a clinical medicine for liver diseases, or chemicals in Herba Artemisiae Scopariae (A. Scopariae), a commonly used traditional Chinese medicine for remedying liver diseases such as hepatitis induced by viruses, chemicals or alcohol. After hepatocyte extraction the compounds were analyzed by HPLC, therefore this method was referrred to as hepatocyte extraction conjugated with HPLC (HE-HPLC). In the first part of this study, HE-HPLC showed that bifendate was extracted by hepatocytes and detected by HPLC-DAD which indicated the feasibility of this method. Then in the second part of the study, the potential active components in the A. scopariae extract were studied using HE-HPLC. Six chemicals in the A. scopariae extract, which could bind to hepatocytes in vitro, were detected by HPLC-DAD and three were identified as 7-hydroxy-coumarin (7-OH-C), capillartemisin A and 7-methoxy-coumarin, respectively. In vitro assays showed that 7-OH-C protected HL-7702 hepatocytes from H2O2 injury. The results indicated that these compounds could be extracted by hepatocytes, could be detected by HPLC and more importantly were bioactive. It is suggested that HE-HPLC is a useful method for screening potent active components in Chinese medicines used to treat liver diseases.
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影响因子：
5
作者：
Pan, Si-Yuan;Yang, Rong;Ko, Kam-Ming
通讯作者：
Ko, Kam-Ming
DOI：
10.1016/j.jchromb.2007.07.040
发表时间：
2007-10-01
影响因子：
3
作者：
Chen, Zhi-Peng;Zhu, Jia-Bi;Xiao, Yan-Yu
通讯作者：
Xiao, Yan-Yu
DOI：
10.1016/s0006-291x(75)80292-0
发表时间：
1975-01-01
影响因子：
3.1
作者：
SEGLEN, O
通讯作者：
SEGLEN, O
影响因子：
5.4
作者：
Chen, Fang-Pey;Kung, Yen-Ying;Hwang, Shinn-Jang
通讯作者：
Hwang, Shinn-Jang
影响因子：
3.3
作者：
Cai, Hui;Song, Yao-Hong;Jin, Miao-Wen
通讯作者：
Jin, Miao-Wen