Detection of exotic QTLs controlling nitrogen stress tolerance among wild barley introgression lines
Detection of exotic QTLs controlling nitrogen stress tolerance among wild barley introgression lines
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检测控制野生大麦渗入系氮胁迫耐受性的外来QTL
DOI：
10.1007/s10681-012-0711-3
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发表时间：
2013
期刊：
影响因子：
1.9
通讯作者：
Pillen K
中科院分区：
文献类型：
--
作者：
Schnaithmann F;Pillen K
氮(N)是最重要的植物营养物质之一，控制着栽培大麦的生长和最终的产量。栽培大麦的野生祖先自养大麦具有提高对生物和非生物胁迫的耐受性的基因。为了在大麦优良品种的遗传背景中定位提高耐氮性的野生大麦等位基因，在温室条件下进行了两个施氮量水平的数量性状基因座(QTL)研究。为此，以大麦杂交组合‘斯嘉丽’和‘ISR42-8’为材料，对28个大麦导入系(S42IL)进行了研究。对包含单个或多个野生大麦导入的S42ILs和‘Scarlett’的15个性状进行了评估，这些性状与形态参数、籽粒参数以及碳(C)和氮含量参数有关。混合模型分析和随后的Dunnett检验被用来识别显著偏离经常性父母‘Scarlett’的S42IL，要么分别针对每个N水平进行测试，要么同时在两个N水平上进行测试。总共检测到65个针对S42IL集合的QTL。抽穗期叶绿素含量的QTL最多(10个)，秸秆C/N比的QTL最少(1个)。个体S42IL具有不同数量的QTL。S42IL-108共检测到8个QTL，而S42IL-145与‘Scarlett’没有显著差异。野生大麦等位基因在32个QTL上表现为减效效应，在33个QTL上表现为增效效应。尽管有25个QTL在两个N水平上表现出相似的效应，但分别有18个和22个QTL表现出仅在N0或N1下检测到的效应。因此，我们认为，在低氮条件下单独选择耐N胁迫的大麦良种是值得的，而不是从标准N肥条件下进行的试验来推断性状表现。一些野生大麦QTL等位基因提高了对N胁迫的耐性。例如，位于4H染色体上的一个野生大麦QTL等位基因存在于S42IL-119的热导入中，它与两个氮素水平下千粒重增加13.0%和低氮供应下千粒重增加20%有关。将本研究检测到的QTL与以往大麦和其他小粒大麦田间研究的QTL进行了比较，并与其他QTL进行了比较。QTL研究之间的一致性(在同一地图位置显示相似效应的QTL)被记录和讨论。根据我们的研究，在S42ILs中有很有前途的野生大麦QTL等位基因，这些等位基因可以很容易地用于大麦育种中提高氮素利用效率的选择。
Nitrogen (N) is one of the most important plant nutrients, controlling growth and, ultimately, yield of a cultivar.Hordeum vulgaressp.spontaneum, the wild barley progenitor of cultivated barley, is known to possess genes that can improve tolerance against biotic and abiotic stresses. A quantitative trait locus (QTL) study with two levels of N fertilization was conducted under glasshouse in order to locate wild barley alleles that improve N stress tolerance in the genetic background of an elite barley cultivar. For this, a set of 28 barley introgression lines (S42ILs), which originate from the cross ‘Scarlett’ × ‘ISR42-8’, was studied. The S42ILs, containing single or multiple wild barley introgressions, and ‘Scarlett’ were evaluated in regard to a total of 15 traits, related to morphological parameters, grain parameters as well as to carbon (C) and N content parameters. A mixed model analysis and a subsequent Dunnett test was conducted to identify S42ILs that significantly deviate from the recurrent parent ‘Scarlett’, either tested separately for each N level, or simultaneously across both N levels. In total, 65 QTLs were detected for the S42IL set. Most QTLs were found for chlorophyll content during heading (10 QTLs) and the fewest for C/N ratio of straw (1 QTL). The individual S42ILs possessed different numbers of QTLs. For S42IL-108, a maximum of eight QTLs were found whereas S42IL-145 did not show any significant difference from ‘Scarlett’. Wild barley alleles revealed decreasing effects at 32 QTLs and increasing effects at 33 QTLs. Although 25 QTLs exhibited similar effects across both N levels, 18 and 22 QTLs exhibited effects that were only detected under N0 or N1, respectively. We, thus, conclude that it may be worth to select improved barley cultivars for N stress tolerance separately under low N fertilization, rather than extrapolating trait performances from experiments carried out under standard N fertilization conditions. A number of wild barley QTL alleles improved N stress tolerance. For example, a wild barley QTL allele on chromosome 4H, present in theHspintrogression of S42IL-119, was associated with a 13.0 % increase of thousand grain weight across both N levels and a 20 % increase under low N supply. QTLs detected in the present study were compared with those of previous field studies of the same cross and with other QTL studies in barley and other small grains. Accordance between QTL studies (QTLs showing similar effects at the same map location) is documented and discussed. Based on our study, promising wild barley QTL alleles are available in S42ILs, which can be readily utilized to select for improved N use efficiency in barley breeding.
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影响因子：
5.4
作者：
M. Korff;J. Léon;K. Pillen
通讯作者：
M. Korff;J. Léon;K. Pillen
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DOI：
--
发表时间：
2004
期刊：
影响因子：
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影响因子：
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作者：
B. Wienhold;J. M. Krupinsky
通讯作者：
J. M. Krupinsky
影响因子：
2
作者：
Bezant, JH;Laurie, DA;Kearsey, MJ
通讯作者：
Kearsey, MJ
DOI：
10.1534/g3.111.000182
发表时间：
2011-08
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Schmalenbach I;March TJ;Bringezu T;Waugh R;Pillen K
通讯作者：
Pillen K