Human IgG response to a salivary peptide, gSG6-P1, as a new immuno-epidemiological tool for evaluating low-level exposure to Anopheles bites.
Human IgG response to a salivary peptide, gSG6-P1, as a new immuno-epidemiological tool for evaluating low-level exposure to Anopheles bites.
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DOI：
10.1186/1475-2875-8-198
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发表时间：
2009-08-13
期刊：
影响因子：
3
通讯作者：
Remoue F
中科院分区：
文献类型：
--
作者：
Poinsignon A;Cornelie S;Ba F;Boulanger D;Sow C;Rossignol M;Sokhna C;Cisse B;Simondon F;Remoue F
疟疾传播率较低的人群尤其面临严重的疟疾发病率和死亡率风险。此外，在接触按蚊病媒程度较低的情况下，用于对按蚊种群进行采样的传统昆虫学方法不够敏感，可能低估了疟疾传播的真实的风险。评估抗体（Ab）反应节肢动物唾液蛋白构成了一种新的工具，估计暴露水平的昆虫叮咬。在疟疾的情况下，最近的一项研究表明，对gSG 6-P1肽的人IgG反应代表了暴露于冈比亚按蚊叮咬的特异性生物标志物。本研究的目的是调查这种生物标志物是否可用于估计个体对按蚊媒介的低水平暴露。在峰和An结束时评价针对gSG 6-P1的IgG Ab水平。冈比亚暴露季节的儿童生活在塞内加尔的村庄，在那里的按蚊密度估计是非常低的经典昆虫诱捕，但疟疾传播发生在研究季节。在接触极低水平按蚊叮咬的儿童中观察到对gSG 6-P1的特异性IgG反应。此外，在特定的IgG抗体水平显着增加，观察到在按蚊暴露季节，而经典的昆虫学数据报告很少或没有按蚊在研究期间。此外，该生物标志物还可用于评价个体暴露的异质性。结果加强了这样的假设，即在暴露季节对gSG 6-P1的IgG应答的评估可以反映真实的人类与嗜热按蚊的接触，并表明这种低暴露的生物标志物可以在个体水平上使用。这种有前途的免疫流行病学标志物可能是一种有用的工具，可以评估在季节性、城市、海拔或旅行者环境中观察到的极低疟疾病媒暴露风险。此外，该生物标志物可用于应用抗媒介策略后的监测调查。
Human populations exposed to low malaria transmission present particular severe risks of malaria morbidity and mortality. In addition, in a context of low-level exposure to Anopheles vector, conventional entomological methods used for sampling Anopheles populations are insufficiently sensitive and probably under-estimate the real risk of malaria transmission. The evaluation of antibody (Ab) responses to arthropod salivary proteins constitutes a novel tool for estimating exposure level to insect bites. In the case of malaria, a recent study has shown that human IgG responses to the gSG6-P1 peptide represented a specific biomarker of exposure to Anopheles gambiae bites. The objective of this study was to investigate if this biomarker can be used to estimate low-level exposure of individuals to Anopheles vector. The IgG Ab level to gSG6-P1 was evaluated at the peak and at the end of the An. gambiae exposure season in children living in Senegalese villages, where the Anopheles density was estimated to be very low by classical entomological trapping but where malaria transmission occurred during the studied season. Specific IgG responses to gSG6-P1 were observed in children exposed to very low-level of Anopheles bites. In addition, a significant increase in the specific IgG Ab level was observed during the Anopheles exposure season whereas classical entomological data have reported very few or no Anopheles during the studied period. Furthermore, this biomarker may also be applicable to evaluate the heterogeneity of individual exposure. The results strengthen the hypothesis that the evaluation of IgG responses to gSG6-P1 during the season of exposure could reflect the real human contact with anthropophilic Anopheles and suggest that this biomarker of low exposure could be used at the individual level. This promising immuno-epidemiological marker could represent a useful tool to assess the risk to very low exposure to malaria vectors as observed in seasonal, urban, altitude or travellers contexts. In addition, this biomarker could be used for the surveillance survey after applying anti-vector strategy.
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影响因子：
--
作者：
PENNEYS, NS;NAYAR, JK;LEONARDI, C
通讯作者：
LEONARDI, C
影响因子：
2.7
作者：
Waitayakul, A;Somsri, S;Udomsangpetch, R
通讯作者：
Udomsangpetch, R
影响因子：
168.9
作者：
Cissé, B;Sokhna, C;Trape, JF
通讯作者：
Trape, JF
DOI：
10.4269/ajtmh.2001.65.219
发表时间：
2001-09-01
影响因子：
3.3
作者：
Nascimento, RJ;Santana, JM;Teixeira, ARL
通讯作者：
Teixeira, ARL
影响因子：
3.3
作者：
Robert, V;Dieng, H;Fontenille, D
通讯作者：
Fontenille, D