Agrobacterium sp. ZX09 β-Glucan Attenuates Enterotoxigenic Escherichia coli-Induced Disruption of Intestinal Epithelium in Weaned Pigs.
Agrobacterium sp. ZX09 β-Glucan Attenuates Enterotoxigenic Escherichia coli-Induced Disruption of Intestinal Epithelium in Weaned Pigs.
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DOI：
10.3390/ijms231810290
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发表时间：
2022-09-07
影响因子：
5.6
通讯作者：
He, Jun
中科院分区：
文献类型：
--
作者：
Zhou, Yuankang;Luo, Yuheng;Yu, Bing;Zheng, Ping;Yu, Jie;Huang, Zhiqing;Mao, Xiangbing;Luo, Junqiu;Yan, Hui;He, Jun
To explore the protective effect of dietary β-glucan (BGL) supplementation on intestinal epithelium exposure to enterotoxigenic Escherichia coli (ETEC), thirty-two weaned pigs were assigned to four groups. Pigs were fed with a basal diet or basal diet containing 500 mg/kg BGL, and were orally infused with ETEC or culture medium. Results showed BGL supplementation had no influence on growth performance in weaned pigs. However, BGL supplementation increased the absorption of D-xylose, and significantly decreased the serum concentrations of D-lactate and diamine oxidase (DAO) in the ETEC-challenged pigs (p < 0.05). Interestingly, BGL significantly increased the abundance of the zonula occludens-1-(ZO-1) in the jejunal epithelium upon ETEC challenge (p < 0.05). BGL supplementation also increased the number of S-phase cells and the number of sIgA-positive cells, but significantly decreased the number of total apoptotic cells in the jejunal epithelium upon ETEC challenge (p < 0.05). Moreover, BGL significantly increased the duodenal catalase (CAT) activity and the ileal total superoxide dismutase (T-SOD) activity in the ETEC-challenged pigs (p < 0.05). Importantly, BGL significantly decreased the expression levels of critical inflammation related proteins such as the tumor necrosis factor-α (TNF-α), interlukin-6 (IL-6), myeloid differentiation factor 88 (MyD88), and nuclear factor-κB (NF-κB) in the jejunal and ileal mucosa upon ETEC challenge (p < 0.05). BGL also elevated the propanoic acid content and the abundance of Lactobacillus and Bacillus in the colon upon ETEC challenge (p < 0.05). These results suggested BGL could alleviate the ETEC-induced intestinal epithelium injury, which may be associated with suppressed inflammation and improved intestinal immunity and antioxidant capacity, as well as the improved intestinal macrobiotic.
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影响因子：
8.7
作者：
Goodridge HS;Wolf AJ;Underhill DM
通讯作者：
Underhill DM
影响因子：
24.1
作者：
Bertheloot D;Latz E;Franklin BS
通讯作者：
Franklin BS
DOI：
10.5402/2012/125864
发表时间：
2012
期刊：
ISRN pharmaceutics
影响因子：
--
作者：
Kofuji K;Aoki A;Tsubaki K;Konishi M;Isobe T;Murata Y
通讯作者：
Murata Y
影响因子：
7
作者：
Duan Q;Chen D;Yu B;Huang Z;Luo Y;Zheng P;Mao X;Yu J;Luo J;Yan H;He J
通讯作者：
He J
影响因子：
7.1
作者：
Isolauri, E;Sütas, Y;Salminen, S
通讯作者：
Salminen, S