Effect of sialyllactose on growth performance and intestinal epithelium functions in weaned pigs challenged by enterotoxigenic Escherichia Coli.
Effect of sialyllactose on growth performance and intestinal epithelium functions in weaned pigs challenged by enterotoxigenic Escherichia Coli.
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DOI：
10.1186/s40104-022-00673-8
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发表时间：
2022-03-03
影响因子：
7
通讯作者：
He J
中科院分区：
文献类型：
--
作者：
Duan Q;Chen D;Yu B;Huang Z;Luo Y;Zheng P;Mao X;Yu J;Luo J;Yan H;He J
唾液酸乳糖（SL）是猪母乳中含量最丰富的低聚糖之一。然而，很少有人知道它对断奶仔猪的生长性能和肠道健康的影响。本研究旨在探讨SL对断奶仔猪肠上皮细胞对产肠毒素大肠杆菌（ETEC）的保护作用。32头猪随机分为4个处理。饲喂基础日粮或含SL（5.0 g/kg）的基础日粮的猪经口输注ETEC或培养基。添加SL可显著提高ETEC攻毒猪的日增重和饲料转化率（P < 0.05）。SL还能显著提高非攻毒猪的干物质（DM）、总能量（GE）和灰分的消化率（P < 0.05）。此外，SL还能显著升高ETEC攻击小鼠血清中免疫球蛋白（伊加、IgG和IgM）的浓度，降低血清中炎性细胞因子（TNF-α、IL-1β和IL-6）的浓度（P < 0.05）。SL显著提高空肠和回肠绒毛高度、绒毛高度与隐窝深度比值（V：C）以及黏膜蔗糖酶和麦芽糖酶活性（P < 0.05）。同时，SL还能显著提高盲肠中微生物代谢产物（如乙酸、丙酸和丁酸）的浓度以及乳酸杆菌、双歧杆菌和芽孢杆菌的丰度（P < 0.05）。SL显著提高ETEC攻击猪空肠小带闭合蛋白-1（ZO-1）、闭合蛋白和脂肪酸转运蛋白-4（FATP-4）的表达水平（P < 0.05）。SL可减轻ETEC攻击后断奶仔猪的炎症和肠道损伤，这与抑制炎性细胞因子的分泌和升高血清免疫球蛋白以及改善肠道上皮功能和微生物群有关。在线版本包含补充材料，可通过10.1186/s40104-022-00673-8获得。
Sialyllactose (SL) is one of the most abundant oligosaccharides present in porcine breast milk. However, little is known about its effect on growth performance and intestinal health in weaned pigs. This study was conducted to explore the protective effect of SL on intestinal epithelium in weaned pigs upon enterotoxigenic Escherichia coli (ETEC) challenge. Thirty-two pigs were randomly divided into four treatments. Pigs fed with a basal diet or basal diet containing SL (5.0 g/kg) were orally infused with ETEC or culture medium. SL supplementation elevated the average daily gain (ADG) and feed efficiency in the ETEC-challenged pigs (P < 0.05). SL also improved the digestibilities of dry matter (DM), gross energy (GE), and ash in non-challenged pigs (P < 0.05). Moreover, SL not only elevated serum concentrations of immunoglobulins (IgA, IgG, and IgM), but also significantly decreased the serum concentrations of inflammatory cytokines (TNF-α, IL-1β, and IL-6) upon ETEC challenge (P < 0.05). Interestingly, SL increased the villus height, the ratio of villus height to crypt depth (V:C), and the activities of mucosal sucrase and maltase in the jejunum and ileum (P < 0.05). SL also elevated the concentrations of microbial metabolites (e.g. acetic acid, propanoic acid, and butyric acid) and the abundance of Lactobacillus, Bifidobacterium, and Bacillus in the cecum (P < 0.05). Importantly, SL significantly elevated the expression levels of jejunal zonula occludins-1 (ZO-1), occluding, and fatty acid transport protein-4 (FATP4) in the ETEC-challenged pigs (P < 0.05). SL can alleviate inflammation and intestinal injury in weaned pigs upon ETEC challenge, which was associated with suppressed secretion of inflammatory cytokines and elevated serum immunoglobulins, as well as improved intestinal epithelium functions and microbiota. The online version contains supplementary material available at 10.1186/s40104-022-00673-8.
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