Morphological Changes in Blood Cells in a Rat Model of Heatstroke: A Pilot Study.
Morphological Changes in Blood Cells in a Rat Model of Heatstroke: A Pilot Study.
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DOI：
10.3390/jcm11164821
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发表时间：
2022-08-17
影响因子：
3.9
通讯作者：
中科院分区：
文献类型：
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尽管随着全球变暖，中暑的威胁越来越大，但病理生理损伤仍然被定义。此外，中暑时外周血细胞的形态学变化还没有得到很好的表征。我们使用39.5 °C气候室评估了大鼠中暑模型中骨髓和血细胞的病理生理变化。孵育3小时后，采集血液和骨髓样品进行形态学检查，并使用延时观察评估体外热对白细胞的直接影响。在致死模型（核心体温超过42.5 ℃）或亚致死模型（<41.5 ℃）中检查血细胞计数和外周/骨髓涂片。在致死模型中出现血小板和白色血细胞计数的显著降低（分别降低>35%和>20%），在亚致死模型中变化较小。观察到血小板凝集，出现大血小板。中性粒细胞常表现为超分叶核，淋巴细胞表现为反应性或胚样改变。此外，热对白细胞的直接影响在41.5 °C下观察到凋亡性细胞死亡，但随后在43 °C下观察到坏死。总之，我们的啮齿动物模型表明，中暑导致血小板聚集，白细胞损伤，和腱膜坏死细胞死亡。这种变化在亚致死性中暑中是温和和可逆的。未成熟细胞的出现可能是由于骨髓微环境的损伤。这些发现可能为潜在的诊断和治疗考虑提供有用的信息。
Despite the increasing threat of heatstroke with global warming, pathophysiologic injury continues to be defined. In addition, morphological changes of the peripheral blood cells in heatstroke have not been well characterized. We evaluated pathophysiologic changes in bone marrow and blood cells in a rat heatstroke model using a 39.5 °C climate chamber. After three hours of incubation, blood and bone marrow samples were collected for morphology, and the direct effects of heat on leukocytes in vitro were evaluated using time-lapse observation. The blood cell count and peripheral/bone marrow smear were examined either in a lethal model (core body temperature exceeded 42.5 °C) or in a sublethal model (<41.5 °C). Significant decreases in platelet and white blood counts occurred in the lethal model (>35% and >20% decreases, respectively) and changes were less in the sublethal model. Platelet clumping with the appearance of large platelets was observed. The neutrophils often demonstrated hyper-segmented nuclei, and lymphocytes showed reactive or blast-like changes. Further, the direct effect of heat on leukocytes noted apoptotic cell death at 41.5 °C, but subsequent necrosis at 43 °C. In summary, our rodent model showed that heatstroke causes platelet aggregation, leukocyte injury, and aponecrotic cell death. Such changes were milder and reversible in sublethal heatstroke. The appearance of immature cells may result from damage to the bone marrow microenvironment. These findings may provide useful information for potential diagnostic and therapeutic considerations.
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影响因子：
3
作者：
Li, Ting Hon Stanford;Wong, Man Sin;Chan, Ching Bill
通讯作者：
Chan, Ching Bill
DOI：
10.1161/atvbaha.107.158709
发表时间：
2008-06-01
影响因子：
8.7
作者：
Roberts, George T.;Ghebeh, Hazem;Bouchama, Abderrezak
通讯作者：
Bouchama, Abderrezak
影响因子：
2.6
作者：
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通讯作者：
Schatzberg, SJ
影响因子：
0.8
作者：
Bruchim, Y.;Loeb, E.;Aroch, I.
通讯作者：
Aroch, I.
影响因子：
1.2
作者：
Riond, Barbara;Weissenbacher, Stefan;Lutz, Hans
通讯作者：
Lutz, Hans