Reconstruction of the carotenoid biosynthetic pathway of Cronobacter sakazakii BAA894 in Escherichia coli.
Reconstruction of the carotenoid biosynthetic pathway of Cronobacter sakazakii BAA894 in Escherichia coli.
复制标题
大肠杆菌中阪崎克罗诺杆菌BAA894类胡萝卜素生物合成途径的重建
DOI：
10.1371/journal.pone.0086739
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Zhang W;Hu X;Wang L;Wang X
坂崎钩吻虫能形成黄色菌落。然而，黄色素的化学结构和生物合成途径尚未确定。本研究对C.坂崎BAA 894进行了纯化和分析。结果表明，黄色素的主要成分为玉米黄质单苷和玉米黄质双苷。一个由7个基因组成的基因簇与C.坂崎菌BAA 894。C.坂崎BAA 894或其部分片段在异源宿主大肠杆菌DH 5 α中进行了重组。这些E.大肠杆菌菌株的分离和分析，通过薄层色谱，紫外可见光谱，高效液相色谱，和电子喷雾电离质谱。这些重新设计的E.大肠杆菌菌株能产生不同种类的类胡萝卜素。E.大肠杆菌菌株均能产生玉米黄嘌呤单糖苷和玉米黄嘌呤双糖苷;表达这7个基因的大肠杆菌菌株可产生番茄红素、β-胡萝卜素、隐黄质和玉米黄质。本研究确定了C.坂崎BAA 894。
Cronobacter sakazakii could form yellow-pigmented colonies. However, the chemical structure and the biosynthetic pathway of the yellow pigments have not been identified. In this study, the yellow pigments of C. sakazakii BAA894 were purified and analyzed. The major components of the yellow pigments were confirmed as zeaxanthin-monoglycoside and zeaxanthin-diglycoside. A gene cluster containing seven genes responsible for the yellow pigmentation in C. sakazakii BAA894 was identified. The seven genes of C. sakazakii BAA894 or parts of them were reconstructed in a heterologous host Escherichia coli DH5α. The pigments formed in these E. coli strains were isolated and analyzed by thin layer chromatography, UV-visible spectroscopy, high performance liquid chromatography, and electron spray ionization-mass spectrometry. These redesigned E. coli strains could produce different carotenoids. E. coli strain expressing all the seven genes could produce zeaxanthin-monoglycoside and zeaxanthin-diglycoside; E. coli strains expressing parts of the seven genes could produce lycopene, β-carotene, cryptoxanthin or zeaxanthin. This study identified the gene cluster responsible for the yellow pigmentation in C. sakazakii BAA894.
登录
查看更多内容
影响因子：
4.2
作者：
Heider SA;Peters-Wendisch P;Wendisch VF
通讯作者：
Wendisch VF
小球藻（Chromochloris）Zofingiensis的番茄红素ε-周期酶基因的分离和表征。通过氮和光调节类胡萝卜素的途径。
影响因子：
5.4
作者：
Cordero BF;Couso I;Leon R;Rodriguez H;Vargas MA
通讯作者：
Vargas MA
影响因子：
--
作者：
Lee, PC;Momen, AZR;Schmidt-Dannert, C
通讯作者：
Schmidt-Dannert, C
影响因子：
2.8
作者：
Seo, Yong Bae;Choi, Seong Seok;Kim, Young Tae
通讯作者：
Kim, Young Tae
影响因子：
3.5
作者：
Tritsch, Denis;Hemmerlin, Andrea;Rohmer, Michel
通讯作者：
Rohmer, Michel