Differential induction of CCL5 by pathogenic and non-pathogenic strains of West Nile virus in brain endothelial cells and astrocytes.
Differential induction of CCL5 by pathogenic and non-pathogenic strains of West Nile virus in brain endothelial cells and astrocytes.
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DOI：
10.1099/vir.0.060558-0
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发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Fredericksen BL
中科院分区：
文献类型：
--
作者：
Hussmann KL;Fredericksen BL
对西尼罗河病毒（WNV）感染的神经炎症反应可以是保护性的，也可以是病理性的，这取决于环境。虽然有几项研究已经检查了WNV感染小鼠脑内的趋化因子谱，但对中枢神经系统（CNS）内的各种细胞类型如何促进趋化因子表达知之甚少。在这里，我们评估了脑微血管内皮细胞和星形胶质细胞中的趋化因子表达，这些细胞包括血脑屏障（BBB）的主要成分，以响应非致病性（WNV-MAD 78）和高致病性（WNV-NY）WNV菌株。与WNV-NY感染的细胞相比，在WNV-MAD 78感染的脑内皮单层中检测到更高水平的趋化因子CCL 5。然而，在WNV感染的星形胶质细胞中观察到相反的特征，表明致病性和非致病性WNV菌株在BBB引起不同的CCL 5特征。因此，包含BBB的细胞可能有助于CNS内随着WNV感染进展而演变的动态促炎反应。
The neuroinflammatory response to West Nile virus (WNV) infection can be either protective or pathological depending on the context. Although several studies have examined chemokine profiles within brains of WNV-infected mice, little is known about how various cell types within the central nervous system (CNS) contribute to chemokine expression. Here, we assessed chemokine expression in brain microvascular endothelial cells and astrocytes, which comprise the major components of the blood–brain barrier (BBB), in response to a non-pathogenic (WNV-MAD78) and a highly pathogenic (WNV-NY) strain of WNV. Higher levels of the chemokine CCL5 were detected in WNV-MAD78-infected brain endothelial monolayers compared with WNV-NY-infected cells. However, the opposite profile was observed in WNV-infected astrocytes, indicating that pathogenic and non-pathogenic strains of WNV provoke different CCL5 profiles at the BBB. Thus, cells comprising the BBB may contribute to a dynamic pro-inflammatory response within the CNS that evolves as WNV infection progresses.
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