aubergine enhances oskar translation in the Drosophila ovary.
aubergine enhances oskar translation in the Drosophila ovary.
复制标题
茄子增强果蝇卵巢中的奥斯卡翻译。
作者：
Joan E. Wilson;Joanne E. Connell;Paul M. Macdonald
虽然母体mRNA的翻译调控对果蝇胚胎的正常发育很重要，但参与这一过程的基因很少。在这篇报告中，我们描述了茄子在奥斯卡翻译中的作用。以前，茄子与背腹图案有关，因为来自茄子突变体母亲的卵是腹侧化的并且很少受精（Schupbach，T.和Wieschaus，E.（1991）Genetics 129，1119-1136）。我们在一种新的遗传筛选中分离出了茄子的两个新等位基因，并表明茄子也是后部身体图案化所必需的，因为来自茄子母亲的受精卵的一小部分发育成缺乏腹部分割的胚胎。虽然茄子突变似乎不影响任何奥斯卡mRNA或蛋白质的稳定性，奥斯卡蛋白质的水平显着降低茄子突变体。因此，茄子是需要加强奥斯卡翻译。虽然茄子依赖性是由oskar 3' UTR中的序列赋予oskar mRNA的，但茄子可能通过与oskar 3' UTR上游序列的相互作用影响oskar翻译。
Although translational regulation of maternal mRNA is important for proper development of the Drosophila embryo, few genes involved in this process have been identified. In this report, we describe the role of aubergine in oskar translation. Previously, aubergine has been implicated in dorsoventral patterning, as eggs from aubergine mutant mothers are ventralized and seldom fertilized (Schupbach, T. and Wieschaus, E. (1991) Genetics 129, 1119-1136). We have isolated two new alleles of aubergine in a novel genetic screen and have shown that aubergine is also required for posterior body patterning, as the small fraction of eggs from aubergine- mothers that are fertilized develop into embryos which lack abdominal segmentation. Although aubergine mutations do not appear to affect the stability of either oskar mRNA or protein, the level of oskar protein is significantly reduced in aubergine mutants. Thus, aubergine is required to enhance oskar translation. While aubergine-dependence is conferred upon oskar mRNA by sequences in the oskar 3' UTR, aubergine may influence oskar translation through an interaction with sequences upstream of the oskar 3' UTR.
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果蝇第二条染色体上的雌性不育突变。
影响因子：
3.3
作者：
Schüpbach,T;Wieschaus,E
通讯作者：
Wieschaus,E
由 exuperantia 基因编码的蛋白质集中在果蝇护士细胞中的 bicoid mRNA 积累位点，但不在卵母细胞或胚胎中。
影响因子：
10.5
作者：
Macdonald,PM;Luk,SK;Kilpatrick,M
通讯作者：
Kilpatrick,M
tudor 蛋白在果蝇胚胎中的分布表明基于定位位点的功能分离。
DOI：
10.1242/dev.119.1.207
发表时间：
1993
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Bardsley,A;McDonald,K;Boswell,RE
通讯作者：
Boswell,RE
影响因子：
10.5
作者：
CHRISTERSON, LB;MCKEARIN, DM
通讯作者：
MCKEARIN, DM