Epidemiological and genetic diversity of Staphylococcus aureus causing bloodstream infection in Shanghai, 2009-2011.
Epidemiological and genetic diversity of Staphylococcus aureus causing bloodstream infection in Shanghai, 2009-2011.
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DOI：
10.1371/journal.pone.0072811
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ni YX
中科院分区：
文献类型：
--
作者：
Chen X;Wang WK;Han LZ;Liu Y;Zhang H;Tang J;Liu QZ;Huangfu YC;Ni YX
金黄色葡萄球菌或耐甲氧西林金黄色葡萄球菌（MRSA）是引起血液感染的重要病原体。本研究旨在调查2009 - 2011年上海市4家医院血液感染患者临床分离的金黄色葡萄球菌的流行病学和遗传多样性。收集了上海市中心城区4家医院引起血液感染的金黄色葡萄球菌分离株。根据临床与实验室标准协会（CLSI）指南对收集到的分离株进行药敏试验，并进行spa型、多位点序列分型、agr型和毒素基因谱分析，以了解分子多样性。此外，MRSA菌株还具有葡萄球菌盒染色体mec （SCCmec）分型特征。利奈唑胺、替柯planin、万古霉素等药物对金黄色葡萄球菌包括MRSA血流感染均有较好的疗效。对甲氧西林敏感的金黄色葡萄球菌（MSSA）在分子特征和毒素基因上表现出明显的多样性，检测到3株含有至少5种毒素的MSSA毒株。发现5株社区相关性MRSA (CA-MRSA)，但在血流感染患者中，大多数（88.7%）MRSA菌株属于两个毒素基因谱不同的流行克隆（ST239-MRSA- III和ST5-MRSA- II）。尽管在医院环境中出现了CA-MRSA菌株，但与医疗保健相关的MRSA （HA-MRSA）菌株仍然是引起血流感染的主要病原体。
Staphylococcus aureus or methicillin-resistant Staphylococcus aureus (MRSA) has been an important pathogen causing bloodstream infections. Our study aimed to investigate the epidemiological and genetic diversity of clinical S. aureus isolates from patients with bloodstream infection in four hospitals of Shanghai from 2009 to 2011. A collection of S. aureus isolates causing bloodstream infection from four hospitals in the central part of Shanghai was carried out. Antimicrobial susceptibility testings of collected isolates were performed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines, and spa-type, multi-locus sequence typing, agr type and toxin gene profiling were performed to explore the molecular diversity. Moreover, MRSA strains were also characterized by Staphylococcal cassette chromosome mec (SCCmec) typing. The drugs such as linezolid, teicoplanin and vancomycin were efficacious for treating S. aureus including MRSA bloodstream infection. Methicillin-sensitive Staphylococcus aureus (MSSA) strains displayed distinct diversity in molecular characterization and toxin genes, and three virulent MSSA strains encoding at least five toxins were detected. Five community-associated MRSA (CA-MRSA) strains were found, but the majority (88.7%) of MRSA strains belonged to two epidemic clones (ST239-MRSA- III and ST5-MRSA- II) with different toxin gene profiles among patients with bloodstream infection. Healthcare-associated MRSA (HA-MRSA) strains were still the main pathogen causing bloodstream infections in spite of the emergence of CA-MRSA strains in hospital setting.
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影响因子：
4.9
作者：
Locke, Jeffrey B.;Morales, Gracia;Stein, Jeffrey L.
通讯作者：
Stein, Jeffrey L.
影响因子：
9.4
作者：
Pérez-Roth, E;Claverie-Martín, F;Méndez-Alvarez, S
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Méndez-Alvarez, S
影响因子：
3.7
作者：
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通讯作者：
Lu, Jang-Jih
影响因子：
11.8
作者：
Liu, Catherine;Bayer, Arnold;Chambers, Henry F.
通讯作者：
Chambers, Henry F.
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4.9
作者：
Kim, James S.;Holtom, Paul;Vigen, Cheryl
通讯作者：
Vigen, Cheryl