Murine Ischemia Reperfusion Without Endotracheal Intubation or Ventilation: A Tutorial.
Murine Ischemia Reperfusion Without Endotracheal Intubation or Ventilation: A Tutorial.
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DOI：
10.1016/j.jacbts.2021.04.007
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发表时间：
2021-07
期刊：
影响因子：
--
通讯作者：
O'Connell TD
中科院分区：
文献类型：
--
作者：
Zhang MJ;Karachenets S;Healy CL;O'Connell TD
心肌梗死（MI）小鼠模型是重要的临床前平台，左冠状动脉（LCA）结扎是诱导小鼠心脏MI的标准方法（1）。尽管传统方法需要在开胸手术前进行气管插管以维持氧合，但在2010年，Gao等人（2，3）引入了一种创新方法来诱导小鼠MI，该方法通过“外化”心脏并快速结扎LCA来避免插管。消除插管的需要减少了气道损伤，气压伤和感染的风险。尽管有这些好处，但在采用方面仍然存在一些技术限制和障碍。他们的报告主要集中在永久性LCA结扎，而不是缺血再灌注（I/R），这是一个更具临床相关性的模型，因为大多数ST段抬高型MI患者现在接受再灌注治疗。此外，也缺乏对雌性小鼠进行手术和系I/R所需的快速释放结的细节。在这里，我们提出了在不插管的情况下进行I/R的技术改进，包括女性特定的细节，并包括一系列该过程的高分辨率视频（图1A，视频1，2和3），以帮助其他研究人员采用这种技术。本研究按照实验室动物护理和使用的公共卫生服务指南进行，所有程序均经明尼苏达大学机构动物护理和使用委员会批准。我们技术的创新包括：1）我们将小鼠头部放置在乳胶包裹的鼻锥内，以在整个手术过程中保持持续的气道正压和氧合（4）; 2）我们将LCA结扎8-0缝线隧道化，
Mouse models of myocardial infarction (MI) are essential preclinical platforms, and left coronary artery (LCA) ligation is the standard method for inducing MI in the mouse heart (1). Although the classical approach requires endotracheal intubation prior to thoracotomy to maintain oxygenation, in 2010, Gao et al (2, 3) introduced an innovative approach to induce MI in the mouse that circumvented the need for intubation by “externalizing” the heart and quickly ligating the LCA. Eliminating the need for intubation reduced airway injury, barotrauma, and infection risk. Despite these benefits, some technical limitations and obstacles to adoption remained. Their report focused primarily on permanent LCA ligation as opposed to ischemia–reperfusion (I/R), which is a more clinically relevant model because most patients presenting with ST-segment elevation MI now receive reperfusion therapy. Furthermore, details for operating on female mice and tying the quick release knot required for I/R were also lacking. Here, we present technical improvements for performing I/R without intubation, include female-specific details, and include a series of highresolution videos of this procedure (Figure 1A, Videos 1, 2, and 3) to aid other researchers in adopting this technique.A full version of our method is included as the Supplemental Appendix. This study was carried out in adherence to the Public Health Service Guide for the Care and Use of Laboratory Animals, and all procedures were approved by the University of Minnesota Institutional Animal Care and Use Committee. Innovations of our technique include that: 1) we place the mouse head inside a latex-wrapped nose cone to maintain continuous positive airway pressure and oxygenation throughout the procedure (4); 2) we tunnel the LCA-ligating 8-0 suture under
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影响因子：
20.1
作者：
Gao E;Lei YH;Shang X;Huang ZM;Zuo L;Boucher M;Fan Q;Chuprun JK;Ma XL;Koch WJ
通讯作者：
Koch WJ
影响因子：
3.6
作者：
Mayer, Catherine A.;Martin, Richard J.;MacFarlane, Peter M.
通讯作者：
MacFarlane, Peter M.
DOI：
10.1007/978-1-62703-505-7_17
发表时间：
2013-01-01
期刊：
WOUND REGENERATION AND REPAIR: METHODS AND PROTOCOLS
影响因子：
--
作者：
Gao, Erhe;Koch, Walter J.
通讯作者：
Koch, Walter J.
DOI：
10.1152/ajpheart.2000.278.4.h1049
发表时间：
2000-04-01
影响因子：
4.8
作者：
Nossuli, TO;Lakshminarayanan, V;Entman, ML
通讯作者：
Entman, ML