Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process.
Efficient conversion of biomass into lipids by using the simultaneous saccharification and enhanced lipid production process.
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通过同时糖化和增强脂质生产过程将生物质有效转化为脂质
DOI：
10.1186/1754-6834-6-36
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发表时间：
2013-03-05
影响因子：
6.3
通讯作者：
Zhao ZK
中科院分区：
文献类型：
--
作者：
Gong Z;Shen H;Wang Q;Yang X;Xie H;Zhao ZK
背景以木质纤维生物质为原料生产微生物油脂在生物柴油生产和生物精炼中具有广阔的应用前景。这通常包括将生物质水解成富含糖的水解物，然后这些水解物被含油微生物用作生产油脂的碳和能源。然而，微生物脂类的成本对于商业化来说仍然高得令人望而却步。更高效和更完整的工艺是微生物油脂技术更好的技术经济性的关键。结果描述了同时糖化和强化油脂生产(SSELP)工艺，该工艺在将纤维素物质转化为脂类方面具有极大的优势，因为它集成了纤维素生物质的水解和油脂的生物合成。具体地说，在营养丰富的培养基中制备的弯曲隐球菌细胞，在没有辅助营养的情况下，在水解酶的存在下，以高剂量接种到生物质悬浮液中生产脂肪。当纤维素用量为32.3g/L时，纤维素转化率、细胞质量、脂肪含量和脂肪系数分别达到98.5%、12.4g/L、59.9%和204.00 mg/g。SSELP工艺的油脂得率高于传统的纤维素单独水解法。离子液体处理玉米秸秆时，纤维素和半纤维素同时消耗。随着时间的推移，没有木糖积累，这表明葡萄糖的影响被规避了。脂肪产量可达112 mg/g再生玉米秸秆。由于没有细菌污染的辅助营养物质，该工艺无需灭菌。结论SSELP工艺促进了木质纤维材料中的纤维素和半纤维素直接转化为微生物脂类。它大大降低了时间和资金成本，同时提高了血脂系数。在不同水平上优化SSELP工艺应进一步提高微生物脂肪技术的效率，进而促进木质纤维生物质生产脂肪酸衍生产品的生物技术生产。
BackgroundMicrobial lipid production by using lignocellulosic biomass as the feedstock holds a great promise for biodiesel production and biorefinery. This usually involves hydrolysis of biomass into sugar-rich hydrolysates, which are then used by oleaginous microorganisms as the carbon and energy sources to produce lipids. However, the costs of microbial lipids remain prohibitively high for commercialization. More efficient and integrated processes are pivotal for better techno-economics of microbial lipid technology.ResultsHere we describe the simultaneous saccharification and enhanced lipid production (SSELP) process that is highly advantageous in terms of converting cellulosic materials into lipids, as it integrates cellulose biomass hydrolysis and lipid biosynthesis. Specifically,Cryptococcus curvatuscells prepared in a nutrient-rich medium were inoculated at high dosage for lipid production in biomass suspension in the presence of hydrolytic enzymes without auxiliary nutrients. When cellulose was loaded at 32.3 g/L, cellulose conversion, cell mass, lipid content and lipid coefficient reached 98.5%, 12.4 g/L, 59.9% and 204 mg/g, respectively. Lipid yields of the SSELP process were higher than those obtained by using the conventional process where cellulose was hydrolyzed separately. When ionic liquid pretreated corn stover was used, both cellulose and hemicellulose were consumed simultaneously. No xylose was accumulated over time, indicating that glucose effect was circumvented. The lipid yield reached 112 mg/g regenerated corn stover. This process could be performed without sterilization because of the absence of auxiliary nutrients for bacterial contamination.ConclusionsThe SSELP process facilitates direct conversion of both cellulose and hemicellulose of lignocellulosic materials into microbial lipids. It greatly reduces time and capital costs while improves lipid coefficient. Optimization of the SSELP process at different levels should further improve the efficiency of microbial lipid technology, which in turn, promote the biotechnological production of fatty acid-derived products from lignocellulosic biomass.
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影响因子：
6.3
作者：
Hu C;Wu S;Wang Q;Jin G;Shen H;Zhao ZK
通讯作者：
Zhao ZK
影响因子：
4.4
作者：
HUANG, L;FORSBERG, CW
通讯作者：
FORSBERG, CW
影响因子：
8.7
作者：
Liang, Yanna;Tang, Tianyu;Umagiliyage, Arosha Loku
通讯作者：
Umagiliyage, Arosha Loku
影响因子：
4.1
作者：
Olofsson, Kim;Rudolf, Andreas;Liden, Gunnar
通讯作者：
Liden, Gunnar
生物去除抑制剂导致皮肤毛孢子菌玉米秸秆水解物脂质发酵过程中脂质产量的提高
影响因子：
11.4
作者：
Huang, Xiao;Wang, Yumei;Bao, Jie
通讯作者：
Bao, Jie