Transcriptome Analysis of Light-Regulated Monoterpenes Biosynthesis in Leaves of Mentha canadensis L.
Transcriptome Analysis of Light-Regulated Monoterpenes Biosynthesis in Leaves of Mentha canadensis L.
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Mentha Canadensis L叶片中光调节的单甲苯生物合成的转录组分析。
DOI：
10.3390/plants10050930
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发表时间：
2021-05-07
期刊：
影响因子：
--
通讯作者：
Liang C
中科院分区：
文献类型：
--
作者：
Yu X;Qi X;Li S;Fang H;Bai Y;Li L;Liu D;Chen Z;Li W;Liang C
光是调节次生代谢合成的关键环境因素。薄荷（Mentha canadensis L.）叶在香料工业和医药中被广泛使用。在弱光处理下，盾形腺毛密度显著降低。GC-MS分析显示精油和薄荷醇含量显著降低。光照影响盾形腺毛的发育和精油产量。然而，这种调节的基本机制是难以捉摸的。为了鉴定光调节油含量变化过程中的关键基因，在24小时黑暗处理和24小时恢复光处理后，收集叶片用于转录组分析。共获得95，579个unigenes，平均长度为754 bp。利用4个公共蛋白质数据库对56.58%的unigenes进行了注释，共发现10,977个差异表达基因（DEGs），涉及光信号通路和单萜合成通路。大多数的TP表现出类似的表达模式：下调后黑暗处理和上调后的光返回。此外，还分析了参与光信号传导途径的基因。一系列响应性转录因子（TF）被鉴定，并可用于代谢工程作为一种有效的策略，提高精油产量。
Light is a key environmental aspect that regulates secondary metabolic synthesis. The essential oil produced in mint (Mentha canadensis L.) leaves is used widely in the aromatics industry and in medicine. Under low-light treatment, significant reductions in peltate glandular trichome densities were observed. GC-MS analysis showed dramatically reduced essential oil and menthol contents. Light affected the peltate glandular trichomes’ development and essential oil yield production. However, the underlying mechanisms of this regulation were elusive. To identify the critical genes during light-regulated changes in oil content, following a 24 h darkness treatment and a 24 h recovery light treatment, leaves were collected for transcriptome analysis. A total of 95,579 unigenes were obtained, with an average length of 754 bp. About 56.58% of the unigenes were annotated using four public protein databases: 10,977 differentially expressed genes (DEGs) were found to be involved in the light signaling pathway and monoterpene synthesis pathway. Most of the TPs showed a similar expression pattern: downregulation after darkness treatment and upregulation after the return of light. In addition, the genes involved in the light signal transduction pathway were analyzed. A series of responsive transcription factors (TFs) were identified and could be used in metabolic engineering as an effective strategy for increasing essential oil yields.
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