Activation of GABAergic Neurons following Tooth Pulp Stimulation
Activation of GABAergic Neurons following Tooth Pulp Stimulation
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牙髓刺激后 GABA 能神经元的激活
DOI：
10.1177/0022034510363231
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发表时间：
2010-03
影响因子：
7.6
通讯作者：
中科院分区：
文献类型：
--
作者：
GABA（γ-氨基丁酸）能神经元在三叉神经脊束核伤害性传递中的功能影响尚未完全确定。采用谷氨酸脱羧酶（GAD）67-绿色荧光蛋白（GFP）基因敲入小鼠和牙髓刺激模型，应用免疫荧光组织化学双标记技术，研究了三叉神经脊束核内GABA能神经元的激活特征。刺激牙髓后2小时，Fos阳性GABA能神经元的数量显著增加。Fos/GFP双标神经元主要分布于刺激同侧三叉神经脊束核极间尾侧过渡亚核（Vi/Vc）和尾侧亚核（Vc）的浅层。随后，双标记神经元的数量逐渐减少，到48小时与对照组相当。我们的研究结果提供了直接的形态学证据，在三叉神经脊束神经系统的GABA能神经元的子集被激活，在牙髓刺激。
The functional impact of GABA (γ-aminobutyric acid)ergic neurons in nociceptive transmission of the spinal trigeminal nucleus is not fully established. Using both the glutamic acid decarboxylase (GAD)67-green fluorescence protein (GFP) knock-in mouse and the tooth pulp stimulation model, we performed double-immunofluorescent histochemistry to determine the characteristics of GABAergic neuron activation in the spinal trigeminal nucleus. The number of Fos-positive GABAergic neuronal profiles was significantly increased 2 hrs after tooth pulp stimulation. The Fos/GFP double-labeled neurons were mainly present in superficial laminae of the spinal trigeminal subnucleus interpolaris-caudalis transition (Vi/Vc) and subnucleus caudalis (Vc) on the side ipsilateral to the stimulation. Subsequently, the number of double-labeled neurons decreased gradually and became comparable with that of the controls by 48 hrs. Our results provide direct morphological evidence that a subset of GABAergic neurons in the spinal trigeminal system was activated during tooth pulp stimulation.
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影响因子：
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