Chlorogenic acid attenuates hydrogen peroxide‑induced oxidative stress in lens epithelial cells.
Chlorogenic acid attenuates hydrogen peroxide‑induced oxidative stress in lens epithelial cells.
复制标题
绿原酸减轻过氧化氢诱导的晶状体上皮细胞氧化应激
DOI：
10.3892/ijmm.2017.3302
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发表时间：
2018-03
影响因子：
5.4
通讯作者：
Bi H
中科院分区：
文献类型：
--
作者：
Song J;Guo D;Bi H
氧化应激在透镜蛋白质降解、氧化、交联和聚集过程中起重要作用，并可引发透镜上皮细胞凋亡。为了研究绿原酸（CGA）对过氧化氢（H2 O2）诱导的氧化应激的保护作用，将人透镜上皮细胞（hLEC）暴露于不同浓度的H2 O2中，并在存在和不存在CGA的情况下进行观察。采用MTT法、RT-qPCR法和ELISA法检测细胞存活率、Bcl-2和Bax蛋白/mRNA水平。此外，细胞内活性氧（ROS）和细胞凋亡的水平，使用流式细胞仪测定CGA对H2 O2诱导的氧化应激的保护作用。此外，CGA对H2 O2诱导的细胞凋亡的保护作用也使用兔晶状体离体检查。结果表明，CGA以剂量依赖性方式降低H2 O2诱导的细胞毒性。流式细胞仪分析表明，同时暴露于H2 O2和CGA显着降低细胞凋亡和ROS水平。RT-qPCR分析显示，在H2 O2暴露24 h后，hLEC中Bcl-2的减少和Bax的增加，无论CGA的存在。ELISA结果显示，CGA处理后24 h，Bcl-2表达增加，Bax表达减少，Bax/Bcl-2比值显著降低。透镜器官培养实验表明，CGA处理后H2 O2诱导的透镜混浊度呈剂量依赖性降低。提示CGA通过Bax/Bcl-2信号通路抑制H2 O2诱导的人晶状体上皮细胞凋亡和透镜混浊。CGA可以提供有效的抗氧化应激的防御，因此在临床实践中具有治疗多种疾病的潜力。
Oxidative stress has an important role in the degradation, oxidation, cross-linking and aggregation of lens proteins, and can trigger lens epithelial cell apoptosis. To investigate the protective effect of chlorogenic acid (CGA) against hydrogen peroxide (H2O2)-induced oxidative stress, human lens epithelial cells (hLECs) were exposed to various concentrations of H2O2 in the presence and absence of CGA. Using MTT assay, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and ELISA techniques, cell viability, and protein/mRNA levels of BCL2 apoptosis regulator (Bcl-2) and BCL2 associated X apoptosis regulator (Bax) were investigated. Additionally, the levels of intracellular reactive oxygen species (ROS) and apoptosis within cells were measured using flow cytometry to determine the protective effect of CGA on H2O2-induced oxidative stress. Furthermore, the protective effect of CGA on H2O2-induced apoptosis was also examined using rabbit lenses ex vivo. The results indicated that CGA reduced H2O2-induced cytotoxicity in a dose-dependent manner. Flow cytometry analysis demonstrated that simultaneous exposure of hLECs to H2O2 and CGA significantly decreased apoptosis and the levels of ROS. RT-qPCR analysis revealed a decrease in Bcl-2 and an increase in Bax in hLECs following exposure to H2O2 for 24 h, regardless of CGA presence. Furthermore, ELISA results indicate that CGA increased Bcl-2 expression and decreased Bax expression following treatment with H2O2 for 24 h and the Bax/Bcl-2 ratio was significantly decreased by CGA treatment. Lens organ culture experiments indicated a dose-dependent decrease in H2O2-induced lens opacity following CGA treatment. These results suggest that CGA suppresses hLECs apoptosis and prevents lens opacity induced by H2O2 via Bax/Bcl-2 signaling pathway. CGA may provide effective defenses against oxidative stress and, thus, haσ potential as treatment for a variety of diseases in clinical practice.
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影响因子：
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作者：
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通讯作者：
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