Human umbilical cord blood-derived CD34+ cells reverse osteoporosis in NOD/SCID mice by altering osteoblastic and osteoclastic activities.
Human umbilical cord blood-derived CD34+ cells reverse osteoporosis in NOD/SCID mice by altering osteoblastic and osteoclastic activities.
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DOI：
10.1371/journal.pone.0039365
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Das H
中科院分区：
文献类型：
--
作者：
Aggarwal R;Lu J;Kanji S;Joseph M;Das M;Noble GJ;McMichael BK;Agarwal S;Hart RT;Sun Z;Lee BS;Rosol TJ;Jackson R;Mao HQ;Pompili VJ;Das H
骨质疏松症是一种与骨矿物质密度和微结构损失相关的骨疾病。成骨细胞和破骨细胞活性的平衡维持骨稳态。由于破骨细胞增加和成骨细胞活性降低而导致的骨质流失增加被认为是骨质疏松症的根本原因。骨质疏松症的治疗方法有限，因此目前正在考虑CD 34+细胞疗法的潜力。我们开发了一种基于纳米纤维的扩增技术，以获得足够数量的从人脐带血中分离的CD 34+细胞，用于治疗应用。在此，我们表明，CD 34+细胞可以分化成成骨细胞系，在体外。全身递送的CD 34+细胞返回骨髓，并显著改善骨质疏松小鼠的骨沉积、骨矿物质密度和骨微结构。血清中骨钙素、IL-10、GM-CSF水平的升高和MCP-1水平的降低与骨微结构的改善平行。此外，CD 34+细胞提高成骨细胞的活性，同时破坏破骨细胞的分化，成熟和功能。这些发现证明了一种利用纳米纤维扩增的CD 34+细胞作为治疗骨质疏松症的治疗应用的新方法。
Osteoporosis is a bone disorder associated with loss of bone mineral density and micro architecture. A balance of osteoblasts and osteoclasts activities maintains bone homeostasis. Increased bone loss due to increased osteoclast and decreased osteoblast activities is considered as an underlying cause of osteoporosis. The cures for osteoporosis are limited, consequently the potential of CD34+ cell therapies is currently being considered. We developed a nanofiber-based expansion technology to obtain adequate numbers of CD34+ cells isolated from human umbilical cord blood, for therapeutic applications. Herein, we show that CD34+ cells could be differentiated into osteoblastic lineage, in vitro. Systemically delivered CD34+ cells home to the bone marrow and significantly improve bone deposition, bone mineral density and bone micro-architecture in osteoporotic mice. The elevated levels of osteocalcin, IL-10, GM-CSF, and decreased levels of MCP-1 in serum parallel the improvements in bone micro-architecture. Furthermore, CD34+ cells improved osteoblast activity and concurrently impaired osteoclast differentiation, maturation and functionality. These findings demonstrate a novel approach utilizing nanofiber-expanded CD34+ cells as a therapeutic application for the treatment of osteoporosis.
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影响因子：
3.3
作者：
Das H;Abdulhameed N;Joseph M;Sakthivel R;Mao HQ;Pompili VJ
通讯作者：
Pompili VJ
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64.5
作者：
Ducy, P;Zhang, R;Karsenty, G
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Karsenty, G
影响因子：
56.9
作者：
Hong, JH;Hwang, ES;Yaffe, MB
通讯作者：
Yaffe, MB
影响因子：
11.8
作者：
Day, TF;Guo, XZ;Yang, YZ
通讯作者：
Yang, YZ
影响因子：
14
作者：
Chua, Kian-Ngiap;Chai, Chou;Mao, Hai-Quan
通讯作者：
Mao, Hai-Quan