Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
Visualisation of chicken macrophages using transgenic reporter genes: insights into the development of the avian macrophage lineage.
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DOI：
10.1242/dev.105593
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Sang HM
中科院分区：
文献类型：
--
作者：
Balic A;Garcia-Morales C;Vervelde L;Gilhooley H;Sherman A;Garceau V;Gutowska MW;Burt DW;Kaiser P;Hume DA;Sang HM
我们已经产生了第一个转基因鸡，其中报告基因表达在一个特定的免疫细胞谱系，基于控制元件的殖民地刺激因子1受体（CSF 1 R）基因座。CSF 1 R内的Fms内含子调控元件（FIRE）在果蝇中高度保守，并且是荧光报告基因的骨髓限制性表达所必需的。在哺乳动物中，CSF 1 R-报告基因特异性地在巨噬细胞谱系的细胞中以高水平表达，而在粒细胞中以低得多的水平表达。报告基因表达的细胞谱系特异性通过与内源性CSF 1 R蛋白的一致表达的证明来证实。在转基因鸟类中，报告基因的表达为巨噬细胞系细胞提供了一个确定的标记，确定了卵黄囊、整个胚胎发育和所有成年组织中的最早阶段。报告基因允许详细和动态可视化的胚胎鸡巨噬细胞。鸡胚巨噬细胞不被招募到切口伤口，但能够识别和吞噬微生物抗原。
We have generated the first transgenic chickens in which reporter genes are expressed in a specific immune cell lineage, based upon control elements of the colony stimulating factor 1 receptor (CSF1R) locus. The Fms intronic regulatory element (FIRE) within CSF1R is shown to be highly conserved in amniotes and absolutely required for myeloid-restricted expression of fluorescent reporter genes. As in mammals, CSF1R-reporter genes were specifically expressed at high levels in cells of the macrophage lineage and at a much lower level in granulocytes. The cell lineage specificity of reporter gene expression was confirmed by demonstration of coincident expression with the endogenous CSF1R protein. In transgenic birds, expression of the reporter gene provided a defined marker for macrophage-lineage cells, identifying the earliest stages in the yolk sac, throughout embryonic development and in all adult tissues. The reporter genes permit detailed and dynamic visualisation of embryonic chicken macrophages. Chicken embryonic macrophages are not recruited to incisional wounds, but are able to recognise and phagocytose microbial antigens.
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