Longitudinal Expression of Testicular TAS1R3 from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs.
Longitudinal Expression of Testicular TAS1R3 from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs.
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DOI：
10.3390/ani11020437
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发表时间：
2021-02-08
期刊：
影响因子：
--
通讯作者：
Mu Q
中科院分区：
文献类型：
--
作者：
Gong T;Wang W;Xu H;Yang Y;Chen X;Meng L;Xu Y;Li Z;Wan S;Mu Q
Taste receptor type 1 subunit 3（T1 R3）是一种甜味/鲜味味觉受体，从舌头到睾丸广泛表达，睾丸表达与雄性不育相关。从江香猪T1 R3在生后发育和生精周期中以阶段依赖的方式表达于伸长精子细胞和间质细胞。T1 R3可能有助于调节精子细胞分化和Leydig细胞功能，因此可能有助于限制各种男性生殖病理的发生。味觉受体1型亚基3（T1 R3），一种甜味/鲜味味觉受体，在小鼠睾丸中的表达与精子发生和类固醇生成有关。以从江香猪为研究对象，探讨睾丸T1 R3在猪出生后发育中的作用。根据睾丸重量、形态和睾酮水平，在出生后15-180天的猪中确定了四个关键发育阶段（青春期前：30天;青春期早期：60天;青春期后期：90天;性成熟：120天）。在发育过程中，睾丸T1 R3表现出阶段依赖性和细胞特异性表达模式。特别是，T1 R3水平从青春期前到青春期显著增加（p < 0.05），并且表达保持高水平直到性成熟（p < 0.05），与磷脂酶Cβ2（PLCβ2）的结果相似。T1 R3/PLCβ2在精子细胞和Leydig细胞的胞质中有较强的表达。在猪生精上皮的8期周期中，T1 R3/PLCβ2在II ~ VI期生精上皮中的表达水平高于其他各期，在伸长/拉长精子细胞和残留体中表达较强。睾丸中味觉受体1型亚基1（T1 R1）的信使RNA（mRNA）水平与T1 R3水平呈相似趋势。这些数据表明T1 R3在调节精子细胞分化和Leydig细胞功能中的可能作用。
Taste receptor type 1 subunit 3 (T1R3), a sweet/umami taste receptor, is widely expressed from the tongue to the testis, and testis expression is associated with male sterility. In Congjiang Xiang pigs, T1R3 is expressed in elongating/elongated spermatids and Leydig cells in a stage-dependent manner during postnatal development and the spermatogenic cycle. T1R3 may contribute to regulation of spermatid differentiation and Leydig cell function, and may therefore help limit the incidence of various male reproductive pathologies. Testicular expression of taste receptor type 1 subunit 3 (T1R3), a sweet/umami taste receptor, has been implicated in spermatogenesis and steroidogenesis in mice. We explored the role of testicular T1R3 in porcine postnatal development using the Congjiang Xiang pig, a rare Chinese miniature pig breed. Based on testicular weights, morphology, and testosterone levels, four key developmental stages were identified in the pig at postnatal days 15–180 (prepuberty: 30 day; early puberty: 60 day; late puberty: 90 day; sexual maturity: 120 day). During development, testicular T1R3 exhibited stage-dependent and cell-specific expression patterns. In particular, T1R3 levels increased significantly from prepuberty to puberty (p < 0.05), and expression remained high until sexual maturity (p < 0.05), similar to results for phospholipase Cβ2 (PLCβ2). The strong expressions of T1R3/PLCβ2 were observed at the cytoplasm of elongating/elongated spermatids and Leydig cells. In the eight-stage cycle of the seminiferous epithelium in pigs, T1R3/PLCβ2 levels were higher in the spermatogenic epithelium at stages II–VI than at the other stages, and the strong expressions were detected in elongating/elongated spermatids and residual bodies. The message RNA (mRNA) levels of taste receptor type 1 subunit 1 (T1R1) in the testis showed a similar trend to levels of T1R3. These data indicate a possible role of T1R3 in the regulation of spermatid differentiation and Leydig cell function.
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影响因子：
3.6
作者：
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通讯作者：
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DOI：
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