The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus.
The transcriptome of the invasive eel swimbladder nematode parasite Anguillicola crassus.
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DOI：
10.1186/1471-2164-14-87
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发表时间：
2013-02-08
期刊：
影响因子：
4.4
通讯作者：
Blaxter M
中科院分区：
文献类型：
--
作者：
Heitlinger E;Bridgett S;Montazam A;Taraschewski H;Blaxter M
厚鳗鲡（Anguillicola crassus）是一种重要的鳗鱼寄生线虫。A. crassus是在东亚，它感染日本鳗鲡，日本鳗鱼。A. 30年前，crassus被引入欧洲鳗鱼Anguilla anguilla。这种寄生虫在其新宿主中的致病性比在其原生宿主中的致病性更高，并且被认为威胁到濒危的An。安圭拉在其范围内。线虫在其新宿主中致病性增加的分子基础尚不清楚。为A.来自Roche 454焦磷酸测序数据的crassus。来自An. japonica和An.过滤欧洲鳗宿主中可能的宿主污染物和核糖体RNA。将剩余的353，055个读段组装成高置信度组装体的11，372个重叠群（跨越6.6 Mb）和较低置信度组装体的另外21，153个单例和重叠群（跨越另外6.2 Mb）。大约55%的高置信度组装重叠群用结构域或蛋白质序列相似性衍生的功能信息进行注释。仅在线虫中保守或A.厚肌更可能具有分泌信号肽。数以千计的高质量的单核苷酸多态性被鉴定，编码多态性与个体线虫之间的差异表达相关。鉴定为正选择的转录物富含肽酶。在欧洲和亚洲A之间差异表达的基因集中富集了参与能量代谢的酶。克拉苏A. crassus的质量很高，将作为今后研究这种重要寄生虫入侵生物学的基础。确定的多态性将提供一个关键的工具集，分析人口结构和基因的鉴定可能参与在欧洲鳗鱼宿主致病性增加。在正选择下鉴定肽酶是该方案的第一步。
Anguillicola crassus is an economically and ecologically important parasitic nematode of eels. The native range of A. crassus is in East Asia, where it infects Anguilla japonica, the Japanese eel. A. crassus was introduced into European eels, Anguilla anguilla, 30 years ago. The parasite is more pathogenic in its new host than in its native one, and is thought to threaten the endangered An. anguilla across its range. The molecular bases for the increased pathogenicity of the nematodes in their new hosts is not known. A reference transcriptome was assembled for A. crassus from Roche 454 pyrosequencing data. Raw reads (756,363 total) from nematodes from An. japonica and An. anguilla hosts were filtered for likely host contaminants and ribosomal RNAs. The remaining 353,055 reads were assembled into 11,372 contigs of a high confidence assembly (spanning 6.6 Mb) and an additional 21,153 singletons and contigs of a lower confidence assembly (spanning an additional 6.2 Mb). Roughly 55% of the high confidence assembly contigs were annotated with domain- or protein sequence similarity derived functional information. Sequences conserved only in nematodes, or unique to A. crassus were more likely to have secretory signal peptides. Thousands of high quality single nucleotide polymorphisms were identified, and coding polymorphism was correlated with differential expression between individual nematodes. Transcripts identified as being under positive selection were enriched in peptidases. Enzymes involved in energy metabolism were enriched in the set of genes differentially expressed between European and Asian A. crassus. The reference transcriptome of A. crassus is of high quality, and will serve as a basis for future work on the invasion biology of this important parasite. The polymorphisms identified will provide a key tool set for analysis of population structure and identification of genes likely to be involved in increased pathogenicity in European eel hosts. The identification of peptidases under positive selection is a first step in this programme.
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影响因子：
3.7
作者：
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通讯作者：
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通讯作者：
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通讯作者：
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作者：
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通讯作者：
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