Betaine supplementations enhance the intestinal immunity of on-growing grass carp (Ctenopharyngodon idella): Partly related to TOR and NF-κB signaling pathways
Betaine supplementations enhance the intestinal immunity of on-growing grass carp (Ctenopharyngodon idella): Partly related to TOR and NF-κB signaling pathways
复制标题
甜菜碱补充剂可增强生长中草鱼（Ctenopharyngodon idella）的肠道免疫力：部分与 TOR 和 NF-κB 信号通路相关
DOI：
10.1016/j.aquaculture.2019.734846
复制
发表时间：
2020-03
期刊：
影响因子：
4.5
通讯作者：
Lin Feng
中科院分区：
文献类型：
--
作者：
Hao Sun;Weidan Jiang;Pei Wu;Yang Liu;Jun Jiang;Qihui Yang;Shengyao Kuang;Ling Tang;Xiaoqiu Zhou;Lin Feng
本研究旨在探讨低蛋氨酸（Met）饲料中添加甜菜碱（BET）对生长草鱼（Ctenopharyngodon idella）生长性能和肠道免疫功能的影响。共540条生长中的草鱼（210 ± 0.68 g）用正常Met饲料和5种含有不同水平（0、1.6、3.2、4.8和6.4 g kg−1）的低Met饲料的BET含量的饲料喂养60 d。随后，通过注射嗜水气单胞菌14天进行激发试验。结果表明，与BET 0组相比，适宜的BET条件（1）可提高鱼抗肠炎能力、肠道溶菌酶活性（LZ）、酸性磷酸酶活性（ACP）、补体C3、C4含量和IgM含量，并上调肝脏表达抗菌肽（LEAP）-2A、LEAP-2B、LEAP-1/hepcidin和β-defensin-1（2）下调肠促炎细胞因子TNF-α、IL-1β、IFN-γ2、IL-6和IL-8的mRNA水平，部分与IKK β、γ/IκBα/NF-κ Bp 65、c-Rel信号通路有关;（3）上调肠道抗炎细胞因子转化生长因子（TGF）-β1、IL-4/13 A和IL-10的mRNA水平，与雷帕霉素（TOR）靶点部分相关[TOR/（S6 K1，4 E-BP 1和4 E-BP 2）]信号通路。BET对生长期草鱼（体重210-776 g）的增重率为113.14%。最后，根据PWG和LZ，估算出生长期草鱼（体重210-776 g）的适宜BET值分别为4.28和4.51 g kg− 1饲料。总体而言，低蛋氨酸饲料中添加BET能提高生长性能，增强肠道免疫功能。
This study aims to explore the effects of dietary betaine (BET) supplementations in low methionine (Met) diet on the growth performance and intestinal immune function of on-growing grass carp (Ctenopharyngodon idella). A total of 540 on-growing grass carp (210 ± 0.68 g) were fed with a normal Met diet and five diets containing graded levels of BET supplementations (0, 1.6, 3.2, 4.8 and 6.4 g kg−1) in low Met diet for 60 days. Subsequently, a challenge test was conducted by injection with Aeromonas hydrophila for 14 days. Results showed that, compared with BET0 group, appropriate BET supplementations (1) could enhance the abilities of fish against enteritis, intestinal lysozyme activities (LZ), acid phosphatase activities (ACP), complement 3 (C3) and C4 contents and IgM contents, and up-regulate the transcript abundances of liver-expressed antimicrobial peptide (LEAP)-2A,LEAP-2B,LEAP-1/hepcidin andβ-defensin-1(P< .05); (2) could down-regulate intestinal pro-inflammatory cytokinesTNF-α,IL-1β,IFN-γ2,IL-6andIL-8mRNA levels partly related to [IKK β, γ/IκBα/NF-κBp65, c-Rel] signaling pathway; (3) could up-regulate intestinal anti-inflammatory cytokine transforming growth factor (TGF)-β1,IL-4/13AandIL-10mRNA levels partly related to the target of rapamycin (TOR) [TOR/(S6K1, 4E-BP1 and 4E-BP2)] signaling pathway. Furthermore, BET could spare part methionine (Met) and the actually comprehensive efficacy of BET relative to DL-Met was estimated to be 113.14% for on-growing grass carp (body weight 210–776 g) based on percent weight gain (PWG). Finally, based on PWG and LZ, the appropriate BET supplementations for on-growing grass carp (body weight 210–776 g) were estimated to be 4.28 and 4.51 g kg−1diet, respectively. In general, dietary BET supplementations in low Met diet boosted the growth performance and enhanced the immune function in the intestines of on-growing grass carp.
登录
查看更多内容
影响因子：
4.7
作者：
Jing Xu;Pei Wu;Wei‐dan Jiang;Yang Liu;Jun Jiang;S. Kuang;Ling Tang;Wuneng Tang;Yong‐An Zhang-Yong‐
通讯作者：
Jing Xu;Pei Wu;Wei‐dan Jiang;Yang Liu;Jun Jiang;S. Kuang;Ling Tang;Wuneng Tang;Yong‐An Zhang-Yong‐
鱼类的粘膜免疫系统：耐受共生体同时对抗病原体的进化。
影响因子：
4.7
作者：
Gomez, Daniela;Sunyer, J. Oriol;Salinas, Irene
通讯作者：
Salinas, Irene
影响因子：
4.4
作者：
TSIAGBE, VK;COOK, ME;SUNDE, ML
通讯作者：
SUNDE, ML
DOI：
10.1152/ajpregu.1995.269.3.r490
发表时间：
1995-09-01
影响因子：
2.8
作者：
CASIROLA, DM;VINNAKOTA, RR;FERRARIS, RP
通讯作者：
FERRARIS, RP
影响因子：
2.5
作者：
M. Sanden;M. Berntssen;Å. Krogdahl;G. Hemre;A. Bakke-McKellep
通讯作者：
M. Sanden;M. Berntssen;Å. Krogdahl;G. Hemre;A. Bakke-McKellep