A Critical Time-Window for the Selective Induction of Hippocampal Memory Consolidation by a Brief Episode of Slow-Wave Sleep.
A Critical Time-Window for the Selective Induction of Hippocampal Memory Consolidation by a Brief Episode of Slow-Wave Sleep.
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通过短暂的慢波睡眠选择性诱导海马记忆巩固的关键时间窗口
DOI：
10.1007/s12264-018-0303-x
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发表时间：
2018-12
影响因子：
5.6
通讯作者：
Yu YQ
中科院分区：
文献类型：
--
作者：
Lu Y;Zhu ZG;Ma QQ;Su YT;Han Y;Wang X;Duan S;Yu YQ
虽然研究广泛，但睡眠在学习和记忆中的确切作用仍然不是很清楚。睡眠剥夺最常用于探索睡眠对学习和记忆的影响，但这些研究的结果不可避免地会因同时存在的压力和痛苦而复杂化。此外，目前还不清楚睡眠和记忆巩固之间是否存在严格的时间窗。在本研究中，我们能够诱导时间锁定的慢波睡眠（SWS）的小鼠通过光遗传学刺激的GABA能神经元在旁区（PZ），提供了一个直接的方法来分析SWS的影响学习和记忆的精确的时间窗口。我们发现，在物体就位任务的训练阶段后立即或15 min光照30 min诱发的SWS显著延长了30 min至6 h的记忆。然而，诱导SWS 30分钟后的培训阶段并没有改善记忆，这表明一个关键的时间窗口之间的诱导短暂发作的SWS和学习记忆巩固。在光刺激期间对小鼠施加轻柔触摸以防止SWS诱导也未能改善记忆，表明SWS在记忆巩固中的特定作用，而不是PZ GABA能神经元本身的激活。光诱导的SWS对Y-迷宫空间记忆和背景恐惧记忆的记忆巩固也有类似的影响，但对线索恐惧记忆没有影响。在测试阶段之前立即进行SWS诱导对记忆表现没有影响，表明SWS不影响记忆提取。因此，通过诱导一个连续发作的SWS，我们揭示了一个关键的时间窗口，为巩固校园依赖的记忆。
Although extensively studied, the exact role of sleep in learning and memory is still not very clear. Sleep deprivation has been most frequently used to explore the effects of sleep on learning and memory, but the results from such studies are inevitably complicated by concurrent stress and distress. Furthermore, it is not clear whether there is a strict time-window between sleep and memory consolidation. In the present study we were able to induce time-locked slow-wave sleep (SWS) in mice by optogenetically stimulating GABAergic neurons in the parafacial zone (PZ), providing a direct approach to analyze the influences of SWS on learning and memory with precise time-windows. We found that SWS induced by light for 30 min immediately or 15 min after the training phase of the object-in-place task significantly prolonged the memory from 30 min to 6 h. However, induction of SWS 30 min after the training phase did not improve memory, suggesting a critical time-window between the induction of a brief episode of SWS and learning for memory consolidation. Application of a gentle touch to the mice during light stimulation to prevent SWS induction also failed to improve memory, indicating the specific role of SWS, but not the activation of PZ GABAergic neurons itself, in memory consolidation. Similar influences of light-induced SWS on memory consolidation also occurred for Y-maze spatial memory and contextual fear memory, but not for cued fear memory. SWS induction immediately before the test phase had no effect on memory performance, indicating that SWS does not affect memory retrieval. Thus, by induction of a brief-episode SWS we have revealed a critical time window for the consolidation of hippocampus-dependent memory.
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影响因子：
25
作者：
Ji, Daoyun;Wilson, Matthew A.
通讯作者：
Wilson, Matthew A.
影响因子：
9.8
作者：
Ribeiro S;Gervasoni D;Soares ES;Zhou Y;Lin SC;Pantoja J;Lavine M;Nicolelis MA
通讯作者：
Nicolelis MA
影响因子：
3.2
作者：
Plihal, W;Born, J
通讯作者：
Born, J
影响因子：
2.7
作者：
Chowdhury, Ananya;Chandra, Ruchi;Jha, Sushi K.
通讯作者：
Jha, Sushi K.
影响因子：
3.7
作者：
Rahman A;Languille S;Lamberty Y;Babiloni C;Perret M;Bordet R;Blin OJ;Jacob T;Auffret A;Schenker E;Richardson J;Pifferi F;Aujard F
通讯作者：
Aujard F