Regional and age-related differences in GAD67 expression of parvalbumin- and calbindin-expressing neurons in the rhesus macaque auditory midbrain and brainstem.
Regional and age-related differences in GAD67 expression of parvalbumin- and calbindin-expressing neurons in the rhesus macaque auditory midbrain and brainstem.
复制标题
恒河猕猴听觉中脑和脑干在GAD67表达的GAD67的区域和年龄相关的差异。
DOI：
10.1002/cne.23659
复制
发表时间：
2014-12-15
影响因子：
2.5
通讯作者：
Recanzone, G. H.
中科院分区：
文献类型：
--
作者：
Gray, D. T.;Engle, J. R.;Rudolph, M. L.;Recanzone, G. H.
表达钙结合蛋白（CaBPs）、小清蛋白（PV）和钙结合蛋白（CB）的神经元在啮齿动物和猕猴的整个听觉系统中都表现出与年龄相关的密度变化。在大脑皮层中，表达这些CaBP的神经元表达GABA能神经传递的标志物，如GAD 67，并且具有充分理解的生理反应特性。最近的证据表明，在啮齿动物听觉脑干，含CaBP的细胞不表达GAD 67。目前还不清楚是否PV和CB含有细胞在皮质下的听觉结构的猕猴同样不表达GAD 67，和更好地了解神经元表达这些蛋白质的神经传递是必要的，在理解与年龄相关的变化，其密度在整个猕猴听觉系统。这是研究与免疫荧光双标记技术，以共同注册的PV和CB表达神经元与GAD 67在上级橄榄复合体和下丘的年轻和老年恒河猴。使用无偏抽样技术计算GAD 67表达PV和CB阳性神经元的比例。我们的研究结果表明，42%和62%的PV和CB阳性神经元在听觉脑干和中脑表达GAD 67，这是显着低于大脑。一般来说，随着年龄的增加，PV+神经元表达GAD 67的数量减少，CB+神经元表达GAD 67的数量增加。这些结果表明，PV和CB表达神经元的抑制分子谱可以随着年龄的变化在皮层下的听觉结构，这些神经元是不同的，从充分描述的抑制性interneurons在大脑皮层中表达这些蛋白质。
Neurons expressing the calcium binding proteins (CaBPs) parvalbumin (PV) and calbindin (CB) have shown age-related density changes throughout the ascending auditory system of both rodents and macaque monkeys. In the cerebral cortex, neurons expressing these CaBPs express markers of GABAergic neurotransmission, such as GAD67, and have well understood physiological response properties. Recent evidence suggests that in the rodent auditory brainstem, CaBP containing cells do not express GAD67. It is unknown whether PV and CB containing cells in subcortical auditory structures of macaques similarly do not express GAD67, and a better understanding of the neurotransmission of neurons expressing these proteins is necessary in understanding the age-related changes in their density throughout the macaque auditory system. This was investigated with immunofluorescent double-labeling techniques to co-register PV and CB expressing neurons with GAD67 in the superior olivary complex and the inferior colliculus of young and aged rhesus macaques. The proportions of GAD67 expressing PV and CB positive neurons were computed using unbiased sampling techniques. Our results indicate that between 42 and 62 percent of PV- and CB-positive neurons in the auditory brainstem and midbrain express GAD67, which is significantly less than in the cerebrum. In general, less PV+ neurons and more CB+ neurons expressed GAD67 as a function of age. These results demonstrate that the inhibitory molecular profile of PV and CB expressing neurons can change with age in subcortical auditory structures, and that these neurons are distinct from the well described inhibitory interneurons which express these proteins in the cerebral cortex.
登录
查看更多内容
影响因子：
4.8
作者：
Engle JR;Gray DT;Turner H;Udell JB;Recanzone GH
通讯作者：
Recanzone GH
影响因子：
3.9
作者：
CASPARY, DM;MILBRANDT, JC;HELFERT, RH
通讯作者：
HELFERT, RH
影响因子：
34.7
作者：
Ascoli, Giorgio A.;Alonso-Nanclares, Lidia;Anderson, Stewart A.;Barrionuevo, German;Benavides-Piccione, Ruth;Burkhalter, Andreas;Buzsaki, Gyoergy;Cauli, Bruno;DeFelipe, Javier;Fairen, Alfonso;Feldmeyer, Dirk;Fishell, Gord;Fregnac, Yves;Freund, Tamas F.;Gardner, Daniel;Gardner, Esther P.;Goldberg, Jesse H.;Helmstaedter, Moritz;Hestrin, Shaul;Karube, Fuyuki;Kisvarday, Zoltan F.;Lambolez, Bertrand;Lewis, David A.;Marin, Oscar;Markram, Henry;Munoz, Alberto;Packer, Adam;Petersen, Carl C. H.;Rockland, Kathleen S.;Rossier, Jean;Rudy, Bernardo;Somogyi, Peter;Staiger, Jochen F.;Tamas, Gabor;Thomson, Alex M.;Toledo-Rodriguez, Maria;Wang, Yun;West, David C.;Yuste, Rafael
通讯作者：
Yuste, Rafael
影响因子：
2.5
作者：
Gray, Daniel T.;Engle, James R.;Recanzone, Gregg H.
通讯作者：
Recanzone, Gregg H.
影响因子：
3.3
作者：
Caspary, DM;Holder, TM;Naritoku, DK
通讯作者：
Naritoku, DK