Acute and chronic in vivo effects of exposure to nicotine and propylene glycol from an E-cigarette on mucociliary clearance in a murine model.
Acute and chronic in vivo effects of exposure to nicotine and propylene glycol from an E-cigarette on mucociliary clearance in a murine model.
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DOI：
10.1080/08958378.2017.1336585
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发表时间：
2017-04
影响因子：
2.1
通讯作者：
McGrath-Morrow SA
中科院分区：
文献类型：
--
作者：
Laube BL;Afshar-Mohajer N;Koehler K;Chen G;Lazarus P;Collaco JM;McGrath-Morrow SA
确定急性（1周）和慢性（3周）暴露于电子烟（E-cig）排放物对小鼠肺中粘膜纤毛清除率（MCC）的影响。c57 bl/6雄性小鼠（年龄10.5 ±2.4周龄）暴露于由Joyetech 510-T®电子烟产生的电子烟气雾剂20分钟/天，所述电子烟气雾剂含有0%尼古丁（N）/丙二醇（PG）1周（n = 6）或3周（n = 9），或2.4%N/PG 1周（n = 6）或3周（n = 9），然后测量MCC。对照小鼠（n = 15）不暴露于单独的PG或N/PG。在吸入99 m锝气溶胶后通过γ照相机评估MCC，并表示为在6小时内从两个肺中去除的放射性的量（MCC 6小时）。测定对照小鼠和暴露于单独PG和N/PG 3周的小鼠的静脉血中的可替宁水平。对照小鼠和急性暴露于单独PG和N/PG的小鼠的MCC 6小时相似，平均值（±1个标准差）分别为8.6± 5.2%、7.5±2.8%和11.2± 5.9%。相比之下，单独长期暴露于PG刺激MCC 6小时（17.2 ±8.0）%，并且该刺激在长期暴露于N/PG（8.7 ±4.6）%后显著减弱（p <0.05）。对照组和单独暴露于PG的小鼠血清可替宁水平<0.5ng/ml，而N/PG暴露组小鼠的平均血清可替宁水平为14.6 ± 12.0ng/ml。在该鼠模型中，与单独暴露于PG相比，每日慢性暴露于N/PG 20 min（但非急性暴露）可减缓MCC，并导致尼古丁的全身吸收。
To determine the effect of an acute (1 week) and chronic (3 weeks) exposure to E-cigarette (E-cig) emissions on mucociliary clearance (MCC) in murine lungs. C57BL/6 male mice (age 10.5 ±2.4 weeks) were exposed for 20min/day to E-cigarette aerosol generated by a Joyetech 510-T® E-cig containing either 0% nicotine (N)/propylene glycol (PG) for 1 week (n = 6), or 3 weeks (n = 9), or 2.4% N/PG for one week (n = 6), or 3 weeks (n = 9), followed by measurement of MCC. Control mice (n = 15) were not exposed to PG alone, or N/PG. MCC was assessed by gamma camera following aspiration of 99mtechnetium aerosol and was expressed as the amount of radioactivity removed from both lungs over 6 hours (MCC6hrs). Venous blood was assayed for cotinine levels in control mice and in mice exposed for 3-weeks to PG alone and N/PG. MCC6hrs in control mice and in mice acutely exposed to PG alone and N/PG was similar, averaging (±1 standard deviation) 8.6±5.2%, 7.5±2.8% and 11.2±5.9%, respectively. In contrast, chronic exposure to PG alone stimulated MCC6hrs (17.2 ±8.0)% and this stimulation was significantly blunted following chronic exposure to N/PG (8.7 ±4.6)% (p < .05). Serum cotinine levels were <0.5ng/ml in control mice and in mice exposed to PG alone, whereas, N/PG exposed mice averaged 14.6 ± 12.0 ng/ml. In this murine model, a chronic, daily, 20 min-exposure to N/PG, but not an acute exposure, slowed MCC, compared to exposure to PG alone and led to systemic absorption of nicotine.
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DOI：
10.1089/jamp.2007.0659
发表时间：
2008-03-01
影响因子：
3.4
作者：
Mall, Marcus A.
通讯作者：
Mall, Marcus A.
影响因子：
3.7
作者：
McGrath-Morrow SA;Hayashi M;Aherrera A;Lopez A;Malinina A;Collaco JM;Neptune E;Klein JD;Winickoff JP;Breysse P;Lazarus P;Chen G
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影响因子：
6
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通讯作者：
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影响因子：
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作者：
Goniewicz, Maciej L.;Lee, Lily
通讯作者：
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影响因子：
4.4
作者：
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