Intravesical chondroitin sulfate inhibits recruitment of inflammatory cells in an acute acid damage "leaky bladder" model of cystitis.
Intravesical chondroitin sulfate inhibits recruitment of inflammatory cells in an acute acid damage "leaky bladder" model of cystitis.
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DOI：
10.1016/j.urology.2011.10.010
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发表时间：
2012-02
期刊：
影响因子：
2.1
通讯作者：
Hurst RE
中科院分区：
文献类型：
--
作者：
Engles CD;Hauser PJ;Abdullah SN;Culkin DJ;Hurst RE
在至少一些患者中，用膀胱内施用的糖胺聚糖（GAG）替代糖胺聚糖（GAG）层是间质性膀胱炎的有效疗法。先前显示膀胱内施用的硫酸软骨素结合至表面受损（“渗漏”）的尿道上皮并恢复其对小离子的不渗透性。本研究调查了“GAG补充疗法”的生理效应是否改变了急性膀胱损伤模型中炎性细胞的募集。用10 mM HCl损伤大鼠膀胱。阴性对照膀胱用PBS处理。第二天，用PBS中的20 mg/mL硫酸软骨素处理动物膀胱，而阴性和阳性对照仅用PBS处理。在用硫酸软骨素处理后2天和4天，将动物安乐死，并通过甲苯胺蓝染色分析其膀胱切片的肥大细胞免疫组织化学标记，使用针对CD-45的抗体对淋巴细胞进行免疫组织化学标记，并使用髓过氧化物酶对中性粒细胞进行免疫组织化学标记。硫酸软骨素治疗统计学显著减少炎症细胞（包括中性粒细胞和肥大细胞）向上皮下空间的募集，但不改变CD-45阳性淋巴细胞的募集。我们第一次证明了膀胱内GAG补充疗法也产生了减少急性损伤膀胱模型中炎性细胞募集的生理作用。这些发现支持使用膀胱内注射的GAG治疗因不透性丧失而导致的膀胱疾病，包括间质性膀胱炎、放射性膀胱炎和化学性膀胱炎，也可能包括其他疾病。
Replacement of the glycosaminoglycan (GAG) layer with intravesically-administered GAGs is an effective therapy for interstitial cystitis in at least some patients. Intravesically-administered chondroitin sulfate was previously shown to bind to and restore the impermeability of surface-damaged (“leaky”) urothelium to small ions. This study investigated whether a physiologic effect of “GAG replenishment therapy” altered recruitment of inflammatory cells in an acute bladder damage model. Rat bladders were damaged with 10mM HCl. Negative control bladders were treated with PBS. On the following day, the animal bladders were treated with 20mg/mL chondroitin sulfate in PBS, while the negative and positive controls were treated with PBS alone. Two and four days after treatment with chondroitin sulfate, animals were euthanized, and sections of their bladders were analyzed by Toluidine Blue staining for mast cells immunohistochemical labeling using antibodies against CD-45 for lymphocytes, and myeloperoxidase for neutrophils. Chondroitin sulfate treatment statistically significantly reduced recruitment of inflammatory cells including neutrophils and mast cells to the suburothelial space but did not alter recruitment of CD-45-positive lymphocytes. For the first time we demonstrate that intravesical GAG replenishment therapy also produces a physiological effect of decreasing recruitment of inflammatory cells in an acute model of damaged bladder. These findings support use of intravesically administered GAG for bladder disorders that result from a loss of impermeability, including interstitial, radiation and chemical cystitis, and possibly others as well.
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影响因子：
2.6
作者：
Rajasekaran, Mahadevan;Stein, Paul;Parsons, C. Lowell
通讯作者：
Parsons, C. Lowell
间质性膀胱炎/膀胱疼痛综合征和膀胱过度活动症的重叠。
DOI：
10.4293/108680810x12674612014743
发表时间：
2010-01
期刊：
JSLS : Journal of the Society of Laparoendoscopic Surgeons
影响因子：
--
作者：
Chung MK;Butrick CW;Chung CW
通讯作者：
Chung CW
影响因子：
2.9
作者：
Kuschert, GSV;Coulin, F;Wells, TNC
通讯作者：
Wells, TNC
影响因子：
2
作者：
PARSONS, CL;STEIN, PC;LEBOW, D
通讯作者：
LEBOW, D
影响因子：
6.6
作者：
Slobodov, G;Feloney, M;Culkin, DJ
通讯作者：
Culkin, DJ