Asymmetrically localized proteins stabilize basal bodies against ciliary beating forces.
Asymmetrically localized proteins stabilize basal bodies against ciliary beating forces.
复制标题
DOI：
10.1083/jcb.201604135
复制
发表时间：
2016-11-21
期刊：
影响因子：
--
通讯作者：
Pearson CG
中科院分区：
文献类型：
--
作者：
Bayless BA;Galati DF;Junker AD;Backer CB;Gaertig J;Pearson CG
基底体（BB）组织并锚定运动的纤毛。这项研究揭示了组件，不对称本地化的旋转对称的BB，在那里他们加强特定的BB域。不对称局部BB组件是抵抗不对称纤毛力所必需的。基底体是放射状对称的、富含微管的结构，其成核和锚动纤毛。纤毛搏动产生不对称的机械力，由基体抵抗。为了抵抗这些力，基体超微结构内的不同区域和微管本身必须稳定。然而，稳定基体的分子组分仍然不清楚。在这里，我们确定Fop 1功能上与已建立的基体稳定性组分Bld 10和Poc 1相互作用。我们发现，Fop 1和微管谷氨酰化纳入基体在不同阶段的组装，最终在他们的不对称富集在特定的三联体微管区域，预计经历最大的机械力从纤毛跳动。Fop 1和微管谷氨酰化都是稳定基底体抵抗纤毛搏动力所必需的。我们的研究表明，微管谷氨酰化和Bld10，Poc1和Fop1稳定的基础机构对纤毛跳动通过不同的但相互依赖的机制产生的力量。
Basal bodies (BBs) organize and anchor motile cilia. This study uncovers components that asymmetrically localize to the rotationally symmetric BBs, where they fortify specific BB domains. Asymmetrically localized BB components are necessary to resist asymmetric ciliary forces. Basal bodies are radially symmetric, microtubule-rich structures that nucleate and anchor motile cilia. Ciliary beating produces asymmetric mechanical forces that are resisted by basal bodies. To resist these forces, distinct regions within the basal body ultrastructure and the microtubules themselves must be stable. However, the molecular components that stabilize basal bodies remain poorly defined. Here, we determine that Fop1 functionally interacts with the established basal body stability components Bld10 and Poc1. We find that Fop1 and microtubule glutamylation incorporate into basal bodies at distinct stages of assembly, culminating in their asymmetric enrichment at specific triplet microtubule regions that are predicted to experience the greatest mechanical force from ciliary beating. Both Fop1 and microtubule glutamylation are required to stabilize basal bodies against ciliary beating forces. Our studies reveal that microtubule glutamylation and Bld10, Poc1, and Fop1 stabilize basal bodies against the forces produced by ciliary beating via distinct yet interdependent mechanisms.
登录
查看更多内容
DOI：
10.1083/jcb.40.3.716
发表时间：
1969-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Allen RD
通讯作者：
Allen RD
影响因子：
5.6
作者：
Mikolajka, Aleksandra;Yan, Xiumin;Holak, Tad A.
通讯作者：
Holak, Tad A.
影响因子：
3.3
作者：
Culver, Brady P.;Meehl, Janet B.;Winey, Mark
通讯作者：
Winey, Mark
DOI：
10.1083/jcb.51.1.286
发表时间：
1971-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dirksen ER
通讯作者：
Dirksen ER
影响因子：
3.3
作者：
Meehl, Janet B.;Bayless, Brian A.;Winey, Mark
通讯作者：
Winey, Mark