Nogo receptor 1 is expressed by nearly all retinal ganglion cells.
Nogo receptor 1 is expressed by nearly all retinal ganglion cells.
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DOI：
10.1371/journal.pone.0196565
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
McGee AW
中科院分区：
文献类型：
--
作者：
Solomon AM;Westbrook T;Field GD;McGee AW
从青光眼到钝力创伤的各种情况都会导致视神经萎缩。识别刺激视神经轴突生长的信号通路可能会导致这些病理的治疗。通过Nogo-66受体1（NgR 1）抑制信号传导促进视神经挤压损伤后轴突的再延伸，而在视网膜神经节细胞（RGC）层和内核层中观察到NgR 1 mRNA和蛋白表达，其中视网膜细胞类型表达NgR 1仍然未知。在这里，我们确定NgR 1在小鼠视网膜的表达模式，通过共同标记的神经元与特定的视网膜神经元的特征标记物与特异性抗体的NgR 1或绿色荧光蛋白表达下的ngr 1启动子的控制。我们证明，超过99%的RGC表达NgR 1。因此，抑制NgR 1功能可以普遍促进RGC的轴突再生。这些结果为NgR 1信号在逆转视神经萎缩中的治疗潜力提供了额外的支持。
A variety of conditions ranging from glaucoma to blunt force trauma lead to optic nerve atrophy. Identifying signaling pathways for stimulating axon growth in the optic nerve may lead to treatments for these pathologies. Inhibiting signaling by the nogo-66 receptor 1 (NgR1) promotes the re-extension of axons following a crush injury to the optic nerve, and while NgR1 mRNA and protein expression are observed in the retinal ganglion cell (RGC) layer and inner nuclear layer, which retinal cell types express NgR1 remains unknown. Here we determine the expression pattern of NgR1 in the mouse retina by co-labeling neurons with characterized markers of specific retinal neurons together with antibodies specific for NgR1 or Green Fluorescent Protein expressed under control of the ngr1 promoter. We demonstrate that more than 99% of RGCs express NgR1. Thus, inhibiting NgR1 function may ubiquitously promote the regeneration of axons by RGCs. These results provide additional support for the therapeutic potential of NgR1 signaling in reversing optic nerve atrophy.
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DOI：
10.1523/jneurosci.0907-11.2011
发表时间：
2011-05-25
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Kay JN;De la Huerta I;Kim IJ;Zhang Y;Yamagata M;Chu MW;Meister M;Sanes JR
通讯作者：
Sanes JR
影响因子：
15.9
作者：
Masland, RH
通讯作者：
Masland, RH
DOI：
10.1073/pnas.1423221112
发表时间：
2015-02-24
影响因子：
11.1
作者：
Baldwin, Katherine T.;Carbajal, Kevin S.;Giger, Roman J.
通讯作者：
Giger, Roman J.
影响因子：
16.2
作者：
Lee, Jae K.;Geoffroy, Cedric G.;Chan, Andrea F.;Tolentino, Kristine E.;Crawford, Michael J.;Leal, Marisa A.;Kang, Brian;Zheng, Binhai
通讯作者：
Zheng, Binhai
影响因子：
3.4
作者：
Galindo-Romero, C.;Aviles-Trigueros, M.;Agudo-Barriuso, M.
通讯作者：
Agudo-Barriuso, M.