Comparative genomics reveals candidate carotenoid pathway regulators of ripening watermelon fruit.
Comparative genomics reveals candidate carotenoid pathway regulators of ripening watermelon fruit.
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DOI：
10.1186/1471-2164-14-781
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发表时间：
2013-11-12
期刊：
影响因子：
4.4
通讯作者：
Lenucci MS
中科院分区：
文献类型：
--
作者：
Grassi S;Piro G;Lee JM;Zheng Y;Fei Z;Dalessandro G;Giovannoni JJ;Lenucci MS
许多水果，包括西瓜，在成熟过程中精通类胡萝卜素的积累。虽然大多数编码类胡萝卜素生物合成途径步骤的基因已被克隆，但迄今为止，这些基因的转录调节因子很少被定义。在这里，我们描述了一组推定的类胡萝卜素相关转录因子的鉴定，这些转录因子来自新鲜西瓜类胡萝卜素，并在果实发育和成熟过程中进行转录组分析。我们的目标是阐明类胡萝卜素途径基因的表达谱，并确定作物改良的候选调节因子和分子靶点。在果实成熟过程中，红熟果实的类胡萝卜素总量逐渐增加，可达55 μg -1 fw。反式番茄红素是对这种增加贡献最大的类胡萝卜素。许多与类胡萝卜素代谢相关的基因在果实成熟过程中表现出表达水平的变化，从而产生了类胡萝卜素合成的代谢通量。番茄红素环化酶基因组成性低表达导致番茄红素积累。通过对西瓜果实发育的RNA-seq表达谱分析，获得了一组表达与成熟和类胡萝卜素积累相关的转录因子。从西瓜和番茄同源类胡萝卜素相关基因中鉴定出19个推测的转录因子基因。其中，6个基因在果实发育和成熟过程中在两种植物的果肉中表达差异。综上所述，这些数据表明，虽然一组共同的代谢基因的调控可能影响西瓜和番茄果实在发育和成熟过程中的类胡萝卜素合成和积累，但在更年期和非更年期果实中，特定的和限制性的调控可能有所不同，这可能与它们在成熟过程中对乙烯的不同敏感性和利用有关。
Many fruits, including watermelon, are proficient in carotenoid accumulation during ripening. While most genes encoding steps in the carotenoid biosynthetic pathway have been cloned, few transcriptional regulators of these genes have been defined to date. Here we describe the identification of a set of putative carotenoid-related transcription factors resulting from fresh watermelon carotenoid and transcriptome analysis during fruit development and ripening. Our goal is to both clarify the expression profiles of carotenoid pathway genes and to identify candidate regulators and molecular targets for crop improvement. Total carotenoids progressively increased during fruit ripening up to ~55 μg g-1 fw in red-ripe fruits. Trans-lycopene was the carotenoid that contributed most to this increase. Many of the genes related to carotenoid metabolism displayed changing expression levels during fruit ripening generating a metabolic flux toward carotenoid synthesis. Constitutive low expression of lycopene cyclase genes resulted in lycopene accumulation. RNA-seq expression profiling of watermelon fruit development yielded a set of transcription factors whose expression was correlated with ripening and carotenoid accumulation. Nineteen putative transcription factor genes from watermelon and homologous to tomato carotenoid-associated genes were identified. Among these, six were differentially expressed in the flesh of both species during fruit development and ripening. Taken together the data suggest that, while the regulation of a common set of metabolic genes likely influences carotenoid synthesis and accumulation in watermelon and tomato fruits during development and ripening, specific and limiting regulators may differ between climacteric and non-climacteric fruits, possibly related to their differential susceptibility to and use of ethylene during ripening.
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影响因子：
13.8
作者：
Enfissi, EMA;Fraser, PD;Bramley, PM
通讯作者：
Bramley, PM
影响因子：
4.8
作者：
Estévez, JM;Cantero, A;León, P
通讯作者：
León, P
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
7.2
作者：
Chung, Mi-Young;Vrebalov, Julia;Giovannoni, James
通讯作者：
Giovannoni, James
影响因子：
6.9
作者：
Cordoba, Elizabeth;Salmi, Mari;Leon, Patricia
通讯作者：
Leon, Patricia