Immunogenicity of recombinant Bacillus subtilis spores expressing Clonorchis sinensis tegumental protein
Immunogenicity of recombinant Bacillus subtilis spores expressing Clonorchis sinensis tegumental protein
复制标题
表达华支睾吸虫皮膜蛋白的重组枯草芽孢杆菌孢子的免疫原性
DOI：
10.1007/s00436-007-0762-x
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发表时间：
2007-10
影响因子：
2
通讯作者：
Huang, Yan
中科院分区：
文献类型：
--
作者：
Hu, Fengyu;Xia, Huimin;Chen, Xiaoxiang;Ma, Changling;Xu, Jin;Zhou, Zhenwen;Hu, Xuchu;Lu, Fangli;Wu, Zhongdao;Yu, Xinbing;Huang, Yan
Clonorchis sinensis, which causes clonorchiasis, is of major socioeconomic importance in China. In this study, we report the use of CotC, a major component of the Bacillus subtilis spore coat, as a fusion partner for the expression of C. sinensis TP20.8 (Tegumental Protein 20.8 kDa) on the spore coat. Western blotting was used to identify TP20.8 surface expression on spores. Recombinant spores displaying the TP20.8 antigen were used for oral immunization and were shown to generate mucosal response in rats. TP20.8-specific secretory IgA in feces reached significant levels 2 weeks after oral dosing. This report shows that surface display of recombinant C. sinensis TP20.8 on B. subtilis spores was immunogenic and B. subtilis spores can be used as a mucosal immunization vehicle for parasite prevention and control.
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影响因子：
4.4
作者：
Duc, LH;Hong, HA;Cutting, SM
通讯作者：
Cutting, SM
DOI：
10.1073/pnas.44.10.1072
发表时间：
1958-01-01
影响因子：
11.1
作者：
SPIZIZEN, J
通讯作者：
SPIZIZEN, J
DOI：
10.1074/jbc.272.7.4509
发表时间：
1997-02
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
H. Roder;R. Fracasso;F. Hoffman;James A. Witowsky;G. Davis;C. Pellegrino
通讯作者：
H. Roder;R. Fracasso;F. Hoffman;James A. Witowsky;G. Davis;C. Pellegrino
影响因子：
2
作者：
Wang, C. R.;Qiu, J. H.;Zhu, X. Q.
通讯作者：
Zhu, X. Q.
影响因子：
5.5
作者：
Mauriello, EMF;Duc, LH;Cutting, SM
通讯作者：
Cutting, SM