RSD1 Is Essential for Stomatal Patterning and Files in Rice.
RSD1 Is Essential for Stomatal Patterning and Files in Rice.
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RSD1 对于水稻的气孔图案和归档至关重要。
DOI：
10.3389/fpls.2020.600021
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发表时间：
2020
影响因子：
5.6
通讯作者：
Hou S
中科院分区：
文献类型：
--
作者：
Yu Q;Chen L;Zhou W;An Y;Luo T;Wu Z;Wang Y;Xi Y;Yan L;Hou S
气孔密度是决定植物气体交换和水分蒸腾效率的重要因素。利用正向遗传学方法，从水稻中筛选到一个气孔密度降低、气孔丛生的水稻气孔发育缺陷1（rsd 1 -1）突变体。在第一次不对称分裂后，一些较大的姐妹细胞进行额外的不对称分裂，产生一个邻近保卫母细胞的小细胞。这些小细胞有的发育成气孔，导致气孔簇集，有的则停滞或发育成铺面细胞。图位克隆后，我们发现该基因编码的蛋白含有DUF 630和DUF 632结构域。进化分析表明DUF 630/632基因家族在陆生植物中分化较早。研究发现，RSD 1基因的缺失会导致气孔发育相关基因表达的紊乱，尤其是气孔密度和分布1（OsSDD 1）基因的表达。通过CRISPR-Cas9构建OsSDD 1缺失突变体，我们发现OsSDD 1突变体与rsd 1突变体相似，气孔密集，气孔附近细胞非常小。OsSDD 1和RSD 1都是抑制异位不对称细胞分裂（ACD）和聚集气孔所必需的。脱水胁迫试验表明，气孔密度的降低增强了rsd 1突变体对脱水的回避能力。本研究主要分析了RSD 1和OsSDD 1在水稻气孔发育中的功能。研究结果为通过调控气孔密度培育抗旱作物提供了理论依据。
Stomatal density is an important factor that determines the efficiency of plant gas exchange and water transpiration. Through forward genetics, we screened a mutant rice stomata developmental defect 1 (rsd1-1) with decreased stomatal density and clustered stomata in rice (Oryza sativa). After the first asymmetric division, some of the larger sister cells undergo an extra asymmetric division to produce a small cell neighboring guard mother cell. Some of these small cells develop into stomata, which leads to stomatal clustering, and the rest arrested or developed into pavement cell. After map-based cloning, we found the protein encoded by this gene containing DUF630 and DUF632 domains. Evolutionary analysis showed that the DUF630/632 gene family differentiated earlier in land plants. It was found that the deletion of RSD1 would lead to the disorder of gene expression regarding stomatal development, especially the expression of stomatal density and distribution 1 (OsSDD1). Through the construction of OsSDD1 deletion mutants by CRISPR-Cas9, we found that, similar to rsd1 mutants, the ossdd1 mutants have clustered stomata and extra small cells adjacent to the stomata. OsSDD1 and RSD1 are both required for inhibiting ectopic asymmetric cell divisions (ACDs) and clustered stomata. By dehydration stress assay, the decreased stomatal density of rsd1 mutants enhanced their dehydration avoidance. This study characterized the functions of RSD1 and OsSDD1 in rice stomatal development. Our findings will be helpful in developing drought-resistant crops through controlling the stomatal density.
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影响因子：
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