The variations of VP1 protein might be associated with nervous system symptoms caused by enterovirus 71 infection.
The variations of VP1 protein might be associated with nervous system symptoms caused by enterovirus 71 infection.
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VP1蛋白变异可能与肠道病毒71感染引起的神经系统症状有关
DOI：
10.1186/1471-2334-14-243
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发表时间：
2014-05-07
影响因子：
3.7
通讯作者：
Zhao W
中科院分区：
文献类型：
--
作者：
Zhang B;Wu X;Huang K;Li L;Zheng L;Wan C;He ML;Zhao W
研究背景肠道病毒71型（EV 71）的VP 1蛋白是一种重要的免疫显性蛋白，负责与宿主受体的结合。然而，VP 1和神经毒力之间的关系仍然知之甚少。本研究旨在探讨EV 71病毒VP 1基因突变与神经毒力表型的关系。他们被分为两组，一组有神经系统症状，另一组没有神经系统症状。结果B基因型与C基因型的神经毒力差异无统计学意义（P> 0.05）。有趣的是，我们发现E145 G/Q、E164 D/K和T292 N/K变异与B基因型的神经系统感染相关。在基因型C的情况下，N31 D突变增加神经并发症的风险，而I262 V突变降低神经并发症的风险。我们使用了一个三维模型的VP 1展示了潜在的分子基础EV 71神经系统tropis.ConclusionsDistinct的变化被证明是与神经毒力表型在不同的基因型。检测基因型和亚型的变异对于预测临床结果可能是重要的。
BackgroundThe VP1 protein of enterovirus 71 (EV71) is an important immunodominant protein which is responsible for host-receptor binding. Nevertheless, the relationship between VP1 and neurovirulence is still poorly understood. In this study, we investigated the relationship between mutation of VP1 and neurovirulent phenotype of EV71 infection.MethodsOne hundred and eighty-seven strains from Genbank were included, with a clear clinical background. They were divided into two groups, one with nervous system symptoms and one with no nervous system symptoms. After alignment, the significance of amino acid variation was determined by using the χ2test and a phylogenetic tree was constructed with MEGA software (version 5.1).ResultsWe showed no significant difference in neurovirulence between genotype B and C. Interestingly, we found that variations of E145G/Q, E164D/K and T292N/K were associated with nervous system infection in genotype B. In the case of genotype C, the N31D mutation increased the risk for nervous complications, whereas I262V mutation decreased the risk of nervous complications. We used a 3D model of VP1 to demonstrate the potential molecular basis for EV71 nervous system tropism.ConclusionsDistinct variations are shown to be associated with neurovirulent phenotype in the different genotype. Detection of variation in genotypes and subtypes may be important for the prediction of clinical outcomes.
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