ABA Receptor Subfamily III Enhances Abscisic Acid Sensitivity and Improves the Drought Tolerance of Arabidopsis.

ABA Receptor Subfamily III Enhances Abscisic Acid Sensitivity and Improves the Drought Tolerance of Arabidopsis.
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ABA 受体亚家族 III 增强拟南芥脱落酸敏感性并提高耐旱性

DOI:
10.3390/ijms19071938
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发表时间:
2018-07-02
影响因子:
5.6
通讯作者:
Yang Y
Yang Y
中科院分区:
生物学2区
文献类型:
--
作者:
Li X;Li G;Li Y;Kong X;Zhang L;Wang J;Li X;Yang Y

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植物激素脱落酸(阿坝)调节植物的生长、发育过程和非生物胁迫。阿坝信号被诱导以响应介导植物对环境挑战(包括高盐和干旱)的适应。ABA结合受体(RCAR/PYR 1/PYL)是ABA信号通路的核心成分,由14个成员组成。在这里,我们观察到RCAR中的三个亚家族在基础和外源阿坝水平上表现出不同的表达模式。随后,我们分别产生过表达亚家族III,RCAR 11-RCAR 14的转基因植物。转基因植株在种子萌发和萌发后的幼苗建立和根长中表现出增加的阿坝敏感性。进一步的研究表明,与野生型植物相比,过表达的亚家族III转基因植物增强了抗旱性,提高了水分利用效率,并加速了胁迫响应基因的表达。这些发现证实了亚家族III在ABA介导的发育过程中起着关键作用,更重要的是,在ABA依赖的途径中参与耐旱性。
The phytohormone abscisic acid (ABA) regulates plant growth, the developmental process, and abiotic stresses. ABA signaling is induced in response to mediate plant acclimation to environmental challenges, including high salinity and drought. The ABA-binding receptors (RCAR/PYR1/PYL), composing of 14 members, are the core components of the ABA-signaling pathway. Here, we observed that the three subfamilies within the RCARs showed different expression patterns at the basal and exogenous ABA levels. Subsequently, we generated transgenic plants overexpressing subfamily III, RCAR11–RCAR14, respectively. The transgenic plants showed increased ABA sensitivity in seed germination and post-germination seedling establishment and root length. Further studies revealed that the overexpressing subfamily III transgenic plants enhanced drought resistance, increased water-use efficiency, and accelerated stress-responsive gene expression compared with the wild-type plants. These findings confirm that the subfamily III plays a key role in ABA-mediated developmental processes and, more importantly, is involved in drought tolerance in the ABA-dependent pathway.
脱甲酸受体的激素信号传导的栅极锁锁机制。
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