Magnetic Nano-Sized SDF-1 Particles Show Promise for Application in Stem Cell-Based Repair of Damaged Tissues.
Magnetic Nano-Sized SDF-1 Particles Show Promise for Application in Stem Cell-Based Repair of Damaged Tissues.
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磁性纳米 SDF-1 颗粒在基于干细胞的受损组织修复中显示出应用前景
DOI：
10.3389/fbioe.2022.831256
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发表时间：
2022
影响因子：
5.7
通讯作者：
Sun, Jianbo
中科院分区：
文献类型：
--
作者：
Wang, Xu;Han, XinXin;Qiu, Yi;Sun, Jianbo
关键词：
基于干细胞的疗法是修复受损组织的一种有前景的选择。干细胞具有归巢特性，在SDF - 1/CXCR4轴的调节下，激活后可被动员到损伤部位。然而，足够水平的干细胞聚集和留存对于确保良好的修复效果至关重要。在干细胞疗法的体内研究中，干细胞递送/招募效率以及在损伤部位的留存相关问题是面临的主要挑战之一。在本研究中，我们设计了一种用于干细胞招募的SDF - 1α磁性纳米颗粒递送系统。我们表达并纯化了一种生物素标记的SDF - 1α蛋白，并通过链霉亲和素 - 生物素连接将其固定在链霉亲和素修饰的磁性纳米颗粒（MNP）上，效率约为14%。SDF - MNP在甘油缓冲液中的物理化学性质与链霉亲和素修饰的MNP相似。进一步的证据表明，即使在125μg/ml MNP的浓度下，SDF - MNP也几乎没有细胞毒性，并且在体外和体内对间充质干细胞都有良好的趋化作用。我们的研究为蛋白质因子的磁性纳米颗粒载体系统的组装提供了一种策略，也为SDF - MNP在基于干细胞的受损骨组织再生疗法中的应用提供了初步证据。
Stem cell-based therapy is a promising option for repair of injured tissue. Stem cells have homing characteristics and can be mobilized to the injury sites following activation, under the regulation of the SDF-1/CXCR4 axis. However, a sufficient level of stem cell aggregation and retention is essential for ensuring favorable repair outcomes. Problems related to stem cell delivery/recruitment efficiency and retention in the injury site are among the main challenges faced during in vivo studies on stem cell therapy. In this study, we designed an SDF-1(alpha) magnetic nanoparticle delivery system for stem cell recruitment. We expressed and purified a biotin-labeled SDF-1(alpha) protein and immobilized it on streptavidin-modified magnetic nanoparticles (MNP) through the streptavidin–biotin linkage, with an efficiency of approximately 14%. The physicochemical properties of the SDF-MNP in glycerol buffer were similar to those of the streptavidin-modified MNP. Further evidence suggested that SDF-MNP barely show cytotoxicity even at a concentration of 125 µg/ml MNP and have a promising chemotaxis effect on mesenchymal stem cells in vitro and in vivo. Our study provides a strategy for the assembly of magnetic nanoparticle carrier systems for protein factors, as well as preliminary evidence for the application of SDF-MNP in stem cell-based therapy for the regeneration of injured bone tissue.
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影响因子：
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作者：
Ho-Shui-Ling A;Bolander J;Rustom LE;Johnson AW;Luyten FP;Picart C
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影响因子：
5.2
作者：
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