Medial septal cholinergic mediation of hippocampal theta rhythm induced by vagal nerve stimulation.
Medial septal cholinergic mediation of hippocampal theta rhythm induced by vagal nerve stimulation.
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DOI：
10.1371/journal.pone.0206532
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Konopacki J
中科院分区：
文献类型：
--
作者：
Broncel A;Bocian R;Kłos-Wojtczak P;Konopacki J
迷走神经电刺激（VNS）多年来一直用于治疗耐药性癫痫患者。这项技术也仍在研究中，作为阿尔茨海默病患者的一种特殊治疗方法。最近我们发现，迷走神经刺激诱导海马结构（HPC）的II型θ节律，这是参与记忆巩固。在本研究中，我们已经扩展了我们以前的观察，并解决了神经基板和药理学概况的HPC II型θ节律诱导的VNS在麻醉大鼠。在雄性Wistar大鼠左迷走神经周围植入VNS袖状电极，钨微电极用于记录HPC场活动，以及内侧隔（MS）套管用于注射局部麻醉剂、普鲁卡因和毒蕈碱剂。一个直接的，短暂的影响，VNS对HPC场电位进行了评估之前和之后的中间隔药物注射。内侧隔注射局麻药普鲁卡因，可逆性地消除了VNS诱导的HPC θ节律。随着胆碱能毒蕈碱激动剂和拮抗剂的使用，我们证明，内侧隔M1受体参与介导的VNS对HPC θ场电位的影响。MS胆碱能M1受体机制不仅整合了来自脑干同步化途径的中枢输入，这是HPC II型θ节律产生的基础，而且还整合了来自脑干中迷走神经传入的输入。
Electrical vagal nerve stimulation (VNS) has been used for years to treat patients with drug-resistant epilepsy. This technique also remains under investigation as a specific treatment of patients with Alzheimer’s disease. Recently we discovered that VNS induced hippocampal formation (HPC) type II theta rhythm, which is involved in memory consolidation. In the present study, we have extended our previous observation and addressed the neuronal substrate and pharmacological profile of HPC type II theta rhythm induced by VNS in anesthetized rats. Male Wistar rats were implanted with a VNS cuff electrode around the left vagus nerve, a tungsten microelectrode for recording the HPC field activity, and a medial septal (MS) cannula for the injection of a local anesthetic, procaine, and muscarinic agents. A direct, brief effect of VNS on the HPC field potential was evaluated before and after medial-septal drug injection. Medial septal injection of local anesthetic, procaine, reversibly abolished VNS-induced HPC theta rhythm. With the use of cholinergic muscarinic agonist and antagonists, we demonstrated that medial septal M1 receptors are involved in the mediation of the VNS effect on HPC theta field potential. The MS cholinergic M1 receptor mechanism integrates not only central inputs from the brainstem synchronizing pathway, which underlies the production of HPC type II theta rhythm, but also the input from the vagal afferents in the brain stem.
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影响因子：
3.8
作者：
BUNO, W;GARCIASANCHEZ, JL;GARCIAAUSTT, E
通讯作者：
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作者：
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