Widely targeted analysis of metabolomic changes of Cucumis sativus induced by cucurbit chlorotic yellows virus.
Widely targeted analysis of metabolomic changes of Cucumis sativus induced by cucurbit chlorotic yellows virus.
复制标题
葫芦褪绿黄化病毒诱导黄瓜代谢组变化的广泛靶向分析
DOI：
10.1186/s12870-022-03555-3
复制
发表时间：
2022-03-31
影响因子：
5.3
通讯作者：
Yan F
中科院分区：
文献类型：
--
作者：
Zhang Z;He H;Yan M;Zhao C;Lei C;Li J;Yan F
背景
植物代谢产物在调节植食性昆虫的行为中起着重要作用。病毒感染可以普遍改变植物的代谢产物，从而操纵昆虫媒介的定向和取食行为，有利于病毒的传播。因此，确定植物在病毒感染后的差异积累的代谢产物可以提供深入了解植物，病毒和昆虫媒介之间的三重相互作用是如何发生的。我们前期的研究发现，媒介烟粉虱在黄瓜花叶病毒感染植株和健康植株上表现出不同的定向行为和表现。瓜类褪绿黄化病毒（Cucurbit chlorotic yellows virus，CCYV）是由B型特异性传播的病毒。烟粉虱处于半持久性状态。在本研究中，我们采取的CCYV，B。以烟粉虱和黄瓜为研究体系，探讨植物代谢产物在三重互作中的作用。
结果
共监测到612种代谢产物在CCYV感染后发生变化。代谢产物主要富含黄酮类、脂类、核苷酸及其衍生物。接种CCYV后7 d，脂类、萜类和黄酮类化合物含量显著降低，氨基酸、核苷酸及其衍生物含量显著增加。在15 dpi时，类黄酮的积累仍然显着减少后，CCYV感染，而脂质，氨基酸，核苷酸和衍生物显着增加。大多数显著增加的代谢物是脂质（溶血磷脂酰乙醇胺，LPE;溶血磷脂酰胆碱，LPC及其异构体）。此外，显著变化的代谢物的数量随着感染期的增加而增加。但只有少数有机酸和酚酸在感染CCYV的黄瓜植株和健康黄瓜植株之间表现出差异。
结论
随着接种时间的延长，CCYV感染抑制了防御性的类黄酮、萜类化合物的代谢，但触发了脂类、氨基酸和核苷酸的代谢。这一结果表明，CCYV感染使黄瓜植株更容易受到粉虱攻击和CCYV感染。防御性化合物的减少和氨基酸的增加可能是提高粉虱对CCYV感染宿主的取食偏好的部分原因。CCYV可以劫持脂质代谢进行病毒复制和组装。
Background
Plant metabolites play vital roles in regulating the behavior of herbivore insects. Virus infection can universally alter plant metabolites to manipulate the orientation and feeding behaviors of insect vector, to favor the transmission of virus. Thus, determining the differentially accumulated metabolites of plant upon virus infection could provide insights into understanding how the triple interactions among plant, virus and insect vector happens. Our previous studies have found that vector whitefly Bemisia tabaci (Gennadius, Hemiptera: Aleyrodidae) showed different orientation behavior and performance on CCYV-infected and healthy cucumber plants. Cucurbit chlorotic yellows virus (CCYV) is exclusively transmitted by B. tabaci in a semi-persistent mode. In this study, we take the CCYV, B. tabaci and cucumber as a research system to explore the functions of phyto-metabolites in the triple interactions.
Results
A total of 612 metabolites changed upon CCYV infection were monitored. Metabolites mainly enriched in flavonoids, lipids, nucleotides and their derivatives. At 7 days post CCYV inoculation (dpi), the contents of lipids, terpenoids and flavonoids remarkably decreased, while amino acids, nucleotides and their derivatives notably up-accumulated. At 15 dpi, the accumulation of flavonoids were still significantly reduced upon CCYV infection, while lipids, amino acids, nucleotides and derivatives were remarkably enhanced. Most of significantly increased metabolites were lipids (lysophosphatidylethanolamine, LPE; lysophosphatidylcholine, LPC and their isomers). Also, the number of significantly changed metabolites increased with the infection period. However, only a few organic acids and phenolic acids showed difference between CCYV-infected and healthy cucumber plants.
Conclusions
CCYV infection repressed the defensive flavonoids, terpeneoids metabolism but triggered the lipids, amino acids and nucleotides metabolism with the inoculation period. This result suggests that CCYV-infection makes cucumber plants more susceptible for whiteflies attack and CCYV infection. The reduction of defensive comounds and the increase of amino acids may be partially responsible for enhancing feeding preference of whiteflies to CCYV-infected hosts. CCYV may hijacked lipid metabolism for virus replication and assembly.
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影响因子：
1.8
作者：
Maluta, N. K. P.;Garzo, E.;Fereres, A.
通讯作者：
Fereres, A.
DOI：
10.3390/v13010137
发表时间：
2021-01-19
期刊：
Viruses
影响因子：
--
作者：
Jiménez J;Moreno A;Fereres A
通讯作者：
Fereres A
一种用于大规模检测、鉴定和定量广泛目标代谢物的新型综合方法：在水稻代谢组学研究中的应用。
影响因子：
27.5
作者：
Chen, Wei;Gong, Liang;Luo, Jie
通讯作者：
Luo, Jie
影响因子：
5.6
作者：
Boncan DAT;Tsang SSK;Li C;Lee IHT;Lam HM;Chan TF;Hui JHL
通讯作者：
Hui JHL
粉虱媒介半持续传播葫芦褪绿黄化病毒的直接证据
影响因子：
4.6
作者：
Li J;Liang X;Wang X;Shi Y;Gu Q;Kuo YW;Falk BW;Yan F
通讯作者：
Yan F