Direct evidence for the semipersistent transmission of Cucurbit chlorotic yellows virus by a whitefly vector.
Direct evidence for the semipersistent transmission of Cucurbit chlorotic yellows virus by a whitefly vector.
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粉虱媒介半持续传播葫芦褪绿黄化病毒的直接证据
DOI：
10.1038/srep36604
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发表时间：
2016-11-04
影响因子：
4.6
通讯作者：
Yan F
中科院分区：
文献类型：
--
作者：
Li J;Liang X;Wang X;Shi Y;Gu Q;Kuo YW;Falk BW;Yan F
瓜类褪绿黄化病毒（Cucurbit chlorotic yellows virus，CCYV）是梭状病毒科（Closteroviridae）蔓状病毒属（Crinivirus）的一种新的植物病毒，目前正在亚洲许多国家蔓延，给瓜类作物造成严重的经济损失。CCYV被认为是由甘薯粉虱，烟粉虱，以半持久性的方式传播。在本研究中，我们提供了直接的证据，CCYV的地中海（MED）的神秘物种的B的半持久性传播。烟粉虱复合体我们研究了CCYV的传播特性，并通过激光共聚焦显微镜免疫荧光标记和定位载体内的病毒保留位点。粉虱成功获得CCYV需要≥1 h的获得进入期（AAP），在AAP 48 h时，RT-PCR阳性粉虱个体比例达到100%。CCYV病毒可在载体中保留12 d，但RT-PCR阳性粉虱比例在3 d时下降到55%。30只粉虱在黄瓜植株上接种24 h后，传毒效率为72.73%。CCYV病毒粒子的保留位点位于病毒粒子饲养载体的前肠。这些结果明确表明了CCYV通过B的半持续传播模式。烟粉虱
Cucurbit chlorotic yellows virus (CCYV) (genus Crinivirus, family Closteroviridae) is an emerging plant virus, and is now spreading and causing severe economic losses to cucurbit crops in many Asian countries. CCYV is believed to be transmitted specifically by the sweetpotato whitefly, Bemisia tabaci, in a semipersistent manner. In the present study, we provide direct evidence for the semipersistent transmission of CCYV by Mediterranean (MED) cryptic species of B. tabaci complex. We investigated CCYV transmission characteristics, and immunofluorescently labeled and localized the virus retention site within the vector by laser confocal microscopy. Whiteflies required ≥1 h of acquisition access period (AAP) to successfully acquire CCYV, and the proportion of RT-PCR positive whitefly individuals reached to 100% at 48 h of AAP. CCYV virons could be retained within vectors as long as 12 d, but the proportion of RT-PCR positive whiteflies dropped to 55% by 3 d. Groups of thirty whiteflies given a 24 h of inoculation access period (IAP) to inoculate CCYV on cucumber plants showed a transmission efficiency rate of 72.73%. The retention site of CCYV virons was located in the foregut of virion-fed vectors. These results definitely indicated the semipersistent transmission mode of CCYV by B. tabaci MED.
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影响因子：
4.5
作者：
Webb, Susan E.;Adkins, Scott;Reitz, Stuart R.
通讯作者：
Reitz, Stuart R.
影响因子：
1.8
作者：
Okuda, Shiori;Okuda, Mitsuru;Iwai, Hisashi
通讯作者：
Iwai, Hisashi
影响因子：
--
作者：
Abrahamian, Peter E.;Abou-Jawdah, Yusuf
通讯作者：
Abou-Jawdah, Yusuf
影响因子：
3.2
作者：
Okuda, Mitsuru;Okazaki, Shinichiro;Sugiyama, Mitsuhiro
通讯作者：
Sugiyama, Mitsuhiro
DOI：
10.1073/pnas.0706608104
发表时间：
2007-11-13
影响因子：
11.1
作者：
Uzest, Marilyne;Gargani, Daniel;Blanc, Stephane
通讯作者：
Blanc, Stephane