The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots.
The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots.
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DOI：
10.3762/bjnano.6.26
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发表时间：
2015
影响因子：
3.1
通讯作者：
Janshoff A
中科院分区：
文献类型：
--
作者：
Breus VV;Pietuch A;Tarantola M;Basché T;Janshoff A
在这项工作中，细胞毒性和细胞阻抗响应的CdSe/ZnS核/壳量子点（QDs）与带正电荷的半胱胺量子点，带负电荷的二氢硫辛酸量子点和两性离子D-青霉胺量子点暴露于狗肾MDCKII细胞进行了比较。用药理学抑制剂预处理细胞表明，纳米颗粒的摄取主要是由于受体独立的途径或羧基化和两性离子QD的自发进入，而对于胺官能化颗粒，可以想象富含胆固醇的膜结构域的参与。发现半胱胺-QD的细胞毒性最小，而D-青霉胺-QD使MDCKII的线粒体活性降低20- 25%。虽然细胞活力似乎不受影响（评估从线粒体活性的变化，使用经典的MTS试验后，24小时的曝光），量子点的结合到细胞内部和它们的运动跨细胞骨架丝（捕获和单粒子跟踪的特点），被证明是妥协的细胞骨架和质膜动力学的完整性，证明了由电细胞基质阻抗传感。
In this work, cytotoxicity and cellular impedance response was compared for CdSe/ZnS core/shell quantum dots (QDs) with positively charged cysteamine–QDs, negatively charged dihydrolipoic acid–QDs and zwitterionic D-penicillamine–QDs exposed to canine kidney MDCKII cells. Pretreatment of cells with pharmacological inhibitors suggested that the uptake of nanoparticles was largely due to receptor-independent pathways or spontaneous entry for carboxylated and zwitterionic QDs, while for amine-functionalized particles involvement of cholesterol-enriched membrane domains is conceivable. Cysteamine–QDs were found to be the least cytotoxic, while D-penicillamine–QDs reduced the mitochondrial activity of MDCKII by 20–25%. Although the cell vitality appeared unaffected (assessed from the changes in mitochondrial activity using a classical MTS assay after 24 h of exposure), the binding of QDs to the cellular interior and their movement across cytoskeletal filaments (captured and characterized by single-particle tracking), was shown to compromise the integrity of the cytoskeletal and plasma membrane dynamics, as evidenced by electric cell–substrate impedance sensing.
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影响因子：
3.4
作者：
Pierobon, Paolo;Achouri, Sarra;Cappello, Giovanni
通讯作者：
Cappello, Giovanni
DOI：
10.1083/jcb.141.4.905
发表时间：
1998-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Orlandi PA;Fishman PH
通讯作者：
Fishman PH
影响因子：
3.9
作者：
Reiss, B;Janshoff, A;Wegener, J
通讯作者：
Wegener, J
影响因子：
17.1
作者：
Breus, Vladimir V.;Heyes, Colin D.;Nienhaus, G. Ulrich
通讯作者：
Nienhaus, G. Ulrich
影响因子：
3.4
作者：
Nelson, Shane R.;Ali, M. Yusuf;Warshaw, David M.
通讯作者：
Warshaw, David M.