The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis
The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis
复制标题
转录因子LcDREB2与LcSAMDC2协同促进羊草的耐盐性
DOI：
10.1007/s11240-012-0264-0
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发表时间：
2013-05
期刊：
影响因子：
--
通讯作者：
Liu gongshe
中科院分区：
文献类型：
--
作者：
Peng xianjun;Zhang lexin;Zhang lixing;Liu zhujiang;Cheng liqin;Yang ying;Shen shihua;Chen shuangyan;Liu gongshe
S-腺苷甲硫氨酸脱羧酶（SAMDC）和脱水反应元件结合蛋白（DREBs）可以提高植物对非生物胁迫的抗性。这些蛋白质已被广泛研究，但SAMDC的转录调控机制仍不清楚。本文从羊草中克隆了LcSAMDC 2基因及其启动子。从LcSAMDC 2的启动子中鉴定出两个DRE顺式元件，并显示其与LcDREB 2结合。亚细胞定位和酵母单杂交实验表明LcDREB 2是一个转录因子。电泳迁移率变动分析（EMSA）显示LcDREB 2可以与含有DRE元件的LcSAMDC 2启动子探针结合。LcDREB 2在L. chinensis愈伤组织中LcSAMDC 2的表达增加。LcDREB 2和LcSAMDC 2启动子在烟草中的共表达激活了GUS活性。这些结果表明LcSAMDC 2是LcDREB 2的下游基因。此外，LcDREB 2和LcSAMDC 2在拟南芥中的转基因表达可以提高转基因株系的耐盐性。这些结果表明LcDREB 2与LcSAMDC 2协同作用有助于植物对非生物胁迫的抗性。
S-Adenosyl-methionine decarboxylase (SAMDC) and dehydration responsive element-binding proteins (DREBs) can improve plant resistance to abiotic stresses. These proteins have been extensively studied, but the mechanism for transcriptional regulation of SAMDC remains unclear. In this paper, the LcSAMDC2 gene and its promoter were isolated from Leymus chinensis. Two DRE cis-elements were identified from the promoter of LcSAMDC2 and shown to bind with LcDREB2. Subcellular localization and yeast one-hybrid assay revealed that LcDREB2 is a transcription factor. An electrophoretic mobility shift assay (EMSA) showed that LcDREB2 can bind to the LcSAMDC2 promoter probe containing a DRE element. Over-expression of LcDREB2 in L. chinensis callus increased expression of LcSAMDC2. Co-expression of LcDREB2 and the promoter of LcSAMDC2 fused with GUS in tobacco activated GUS activity. These results indicate that LcSAMDC2 is the downstream gene of LcDREB2. In addition, transgenic expression of LcDREB2 and LcSAMDC2 in Arabidopsis can improve the salt stress tolerance of transgenic lines. These results indicate that LcDREB2 cooperating with LcSAMDC2 contributes to resistance to abiotic stress.
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影响因子：
5.1
作者：
T. Dresselhaus;P. Barceló;C. Hagel;H. Lörz;K. Humbeck
通讯作者：
T. Dresselhaus;P. Barceló;C. Hagel;H. Lörz;K. Humbeck
DOI：
10.1007/s11240-010-9906-2
发表时间：
2011-08
期刊：
Plant Cell, Tissue and Organ Culture (PCTOC)
影响因子：
--
作者：
A. Khedr;M. Serag;Mamdouh M. Nemat-Alla;Amina Z. Abo-Elnaga;R. Nada;W. P. Quick;G. Abogadallah
通讯作者：
A. Khedr;M. Serag;Mamdouh M. Nemat-Alla;Amina Z. Abo-Elnaga;R. Nada;W. P. Quick;G. Abogadallah
影响因子：
5.4
作者：
Ai-Guo Tian;Jingyan Zhao;Jin‐Song Zhang;J. Gai;S. Chen
通讯作者：
Ai-Guo Tian;Jingyan Zhao;Jin‐Song Zhang;J. Gai;S. Chen
影响因子：
3
作者：
Alvarez, I;Tomaro, ML;Benavides, MP
通讯作者：
Benavides, MP
影响因子：
3
作者：
Hu, You-zhen;Zeng, You-ling;Zhang, Fu-chun
通讯作者：
Zhang, Fu-chun