VEGF promotes osteogenic differentiation of ASCs on ordered fluorapatite surfaces.
VEGF promotes osteogenic differentiation of ASCs on ordered fluorapatite surfaces.
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DOI：
10.1002/jbm.a.35215
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发表时间：
2015-02
影响因子：
4.9
通讯作者：
Liu, J.
中科院分区：
文献类型：
--
作者：
Clark, D.;Wang, X.;Chang, S.;Czajka-Jakubowska, A.;Clarkson, B. H.;Liu, J.
血管内皮生长因子（VEGF）已被报道在骨再生中介导骨生成和血管生成。我们之前发现，当新型氟磷灰石（FA）涂层表面发生明显矿化时，脂肪源性干细胞（ASC）中VEGF的上调。本研究探讨了FA和VEGF对有序FA表面生长的ASC生长、分化和矿化的影响。在FA上生长并经VEGF处理的细胞ALP染色显示出成骨分化，茜素红染色显示出明显的矿化。用两种指标观察FA和VEGF的联合刺激作用。使用特定的VEGF受体抑制剂干扰VEGF信号通路显示ALP和茜素红染色水平最低，当细胞生长在FA和/或添加VEGF处理时，ALP和茜素红染色部分恢复。这些FA表面和VEGF刺激ASCs的成骨分化已被证明是通过（但可能不仅仅是）VEGF信号通路介导的。增强成骨分化和矿化支持治疗性VEGF和FA涂层在骨再生中的潜在应用。
Vascular endothelial growth factor (VEGF) has been reported to mediate both osteogenesis and angiogenesis in bone regeneration. We previously found an up regulation of VEGF in adipose-derived stem cells (ASC) when obvious mineralization occurred on a novel fluorapatite (FA) coated surfaces. This study investigated the effect of FA and VEGF on the growth, differentiation and mineralization of (ASC) grown on ordered FA surfaces. Cells grown on FA and treated with VEGF demonstrated osteogenic differentiation as measured with ALP staining, and obvious mineralization as measured by Alizarin Red staining. A combined stimulating effect of FA and VEGF was seen using both indicators. VEGF signaling pathway perturbation using a specific VEGF receptor inhibitor showed the lowest levels of ALP and Alizarin Red staining, which was partially rescued when the cells were grown on FA and/or treated with the addition of VEGF. The osteogenic differentiation of ASCs stimulated by these FA surfaces as well as VEGF has been shown to be mediated through, but probably not only, the VEGF signaling pathway. The enhancement of osteogenic differentiation and mineralization supports the potential use of therapeutic VEGF and FA coatings in bone regeneration.
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血管组织是在分散骨质发生引起的骨形成过程中BMP2表达的主要来源。
影响因子：
4.1
作者：
Matsubara, Hidenori;Hogan, Daniel E.;Morgan, Elise F.;Mortlock, Douglas P.;Einhorn, Thomas A.;Gerstenfeld, Louis C.
通讯作者：
Gerstenfeld, Louis C.
影响因子：
3
作者：
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通讯作者：
Casals, Nuria
影响因子：
5.8
作者：
Morabito, Alessandro;De Maio, Ermelinda;Perrone, Francesco
通讯作者：
Perrone, Francesco
影响因子：
4.1
作者：
Liu, Jun;Jin, Taocong;Clarkson, Brian H.
通讯作者：
Clarkson, Brian H.
DOI：
10.1023/a:1008901512273
发表时间：
2000-12-01
影响因子：
3.7
作者：
Fazan, F;Marquis, PM
通讯作者：
Marquis, PM