Application of Fourier transform-second-harmonic generation imaging to the rat cervix.

Application of Fourier transform-second-harmonic generation imaging to the rat cervix.
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DOI:
10.1111/jmi.12046
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发表时间:
2013-07
影响因子:
2
通讯作者:
Toussaint KC Jr
Toussaint KC Jr
中科院分区:
工程技术4区
文献类型:
--
作者:
Lau TY;Sangha HK;Chien EK;McFarlin BL;Wagoner Johnson AJ;Toussaint KC Jr

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本文应用傅立叶变换二次谐波成像技术对5只非妊娠大鼠子宫颈胶原纤维的排列进行了研究。在二维(2D)和三维(3D)中分析来自宫颈中部和宫颈外口附近的组织切片。我们验证了可以使用FT-SHG成像在三维空间中定量评估宫颈微结构,并观察最外层和最内层中平面内和平面外的胶原纤维,这不能单独使用2D FT-SHG分析观察到。这种方法有可能成为一种非侵入性的方法,用于测量人类宫颈重塑过程中胶原组织的渐进性变化。
We present the application of Fourier transform-second-harmonic generation (FT-SHG) imaging to evaluate the arrangement of collagen fibers in five non-pregnant rat cervices. Tissue slices from the mid-cervix and near the external orifice of the cervix were analyzed in both two-dimensions (2D) and three-dimensions (3D). We validate that the cervical microstructure can be quantitatively assessed in three dimensions using FT-SHG imaging, and observe collagen fibers oriented both in and out-of-plane in the outermost and the innermost layers, which cannot be observed using 2D FT-SHG analysis alone. This approach has the potential to be a noninvasive method for measuring progressive changes in collagen organization during cervical remodeling in humans.
妊娠期间宫颈基质的机械性能与细胞外基质成分之间的关​​系。
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