An electrochemical sensor for the detection of protein-small molecule interactions directly in serum and other complex matrices.
An electrochemical sensor for the detection of protein-small molecule interactions directly in serum and other complex matrices.
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DOI：
10.1021/ja9011595
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发表时间：
2009-05-27
影响因子：
15
通讯作者：
Plaxco KW
中科院分区：
文献类型：
--
作者：
Cash KJ;Ricci F;Plaxco KW
在这里，我们已经证明了一个通用的，敏感的，和选择性的方法，用于检测结合到特定的小分子识别元件的大分子。我们的电化学方法利用氧化还原标记的DNA信号传导支架，该支架与小分子识别元件缀合并共价连接到询问电极。蛋白质与小分子识别元件的结合改变了支架的动力学，增加或降低了氧化还原标签与电极碰撞的效率，从而改变了观察到的法拉第电流。我们使用生物素识别元件和链霉亲和素作为目标来优化支架，以确定定义传感器性能的变量，然后将该方法应用于使用类固醇作为识别元件检测抗地高辛抗体。我们生成了表现出信号开启（目标结合增加法拉第电流）和信号关闭行为的链霉亲和素传感器，其中只有信号关闭方法可推广到抗体检测。这两种目标的传感器都是敏感的（检测限在低纳摩尔范围内），快速（分钟），可重复使用，并具有足够的选择性，可以直接在复杂的基质中发挥作用，包括血清，土壤和食品。
Here we have demonstrated a general, sensitive, and selective approach for the detection of macromolecules that bind to specific small molecule recognition elements. Our electrochemical approach utilizes a redox-tagged DNA signaling scaffold that is conjugated to a small molecule recognition element and is covalently attached to an interrogating electrode. The binding of a protein to the small molecule recognition element alters the dynamics of the scaffold, increasing or decreasing the efficiency with which the redox tag collides with the electrode and thus altering the observed faradaic current. We optimized the scaffold using a biotin recognition element and streptavidin as a target to determine the variables that define sensor performance before then applying the approach to detection of anti-digoxigenin antibodies using the steroid as the recognition element. We generated streptavidin sensors exhibiting both signal-on (target binding increases the faradaic current) and signal-off behavior, of which only the signal-off approach was generalizable to the detection of antibodies. Sensors for both targets are sensitive (detection limits in the low nanomolar range), rapid (minutes), reusable, and selective enough to function directly in complex matrices including blood serum, soil, and foodstuffs.
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影响因子：
4.9
作者：
Ricci, Francesco;Lai, Rebecca Y.;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
影响因子：
15
作者：
Radi, AE;Sánchez, JLA;O'Sullivan, CK
通讯作者：
O'Sullivan, CK
影响因子：
7.4
作者：
Lubin, Arica A.;Lai, Rebecca Y.;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
DOI：
10.1073/pnas.1633515100
发表时间：
2003-08-05
影响因子：
11.1
作者：
Fan, CH;Plaxco, KW;Heeger, AJ
通讯作者：
Heeger, AJ
影响因子：
10.8
作者：
Rant, Ulrich;Pringsheim, Erika;Abstreiter, Gerhard
通讯作者：
Abstreiter, Gerhard