A novel expansin protein from the white-rot fungus Schizophyllum commune.

A novel expansin protein from the white-rot fungus Schizophyllum commune.
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DOI:
10.1371/journal.pone.0122296
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发表时间:
2015
期刊:
影响因子:
3.7
通讯作者:
Folch-Mallol JL
Folch-Mallol JL
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Tovar-Herrera OE;Batista-García RA;Sánchez-Carbente Mdel R;Iracheta-Cárdenas MM;Arévalo-Niño K;Folch-Mallol JL

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从担子真菌裂菌属(Schizophylum commune)中克隆并表达了一个新的扩展蛋白(ScExlx1)。该蛋白具有植物扩增蛋白的典型特征。ScExlx1显示出在棉纤维中形成“气泡”的能力,减少了细轴颗粒的大小,并提高了用该蛋白预处理的棉纤维的还原糖的释放,然后用纤维素酶处理。ScExlx1能与纤维素、桦木木聚糖和几丁质结合,且不受不同氯化钠浓度的影响。ScExlx1的一个新特性是它能够增强从预处理几丁质中释放还原糖(n-乙酰氨基葡萄糖)的能力,并进一步添加几丁质酶,这在任何扩张蛋白或扩张蛋白样蛋白中都没有报道。据我们所知,这是第一次在担子菌中发现真正的真菌扩张蛋白,我们可以在毕赤酵母中表达这种生物活性蛋白。
A novel expansin protein (ScExlx1) was found, cloned and expressed from the Basidiomycete fungus Schizophylum commune. This protein showed the canonical features of plant expansins. ScExlx1 showed the ability to form “bubbles” in cotton fibers, reduce the size of avicel particles and enhance reducing sugar liberation from cotton fibers pretreated with the protein and then treated with cellulases. ScExlx1 was able to bind cellulose, birchwood xylan and chitin and this property was not affected by different sodium chloride concentrations. A novel property of ScExlx1 is its capacity to enhance reducing sugars (N-acetyl glucosamine) liberation from pretreated chitin and further added with chitinase, which has not been reported for any expansin or expansin-like protein. To the best of our knowledge, this is the first report of a bona fide fungal expansin found in a basidiomycete and we could express the bioactive protein in Pichia pastoris.
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