Human cells with osteogenic potential in bone tissue research.
Human cells with osteogenic potential in bone tissue research.
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DOI：
10.1186/s12938-023-01096-w
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发表时间：
2023-04-03
影响因子：
3.9
通讯作者：
Babuska, Vaclav
中科院分区：
文献类型：
--
作者：
Dvorakova, Jana;Wiesnerova, Lucie;Chocholata, Petra;Kulda, Vlastimil;Landsmann, Lukas;Cedikova, Miroslava;Kripnerova, Michaela;Eberlova, Lada;Babuska, Vaclav
关键词：
损伤后或因疾病而手术切除骨后的骨再生是一个严重的医学挑战。正在测试各种材料来替代缺失的骨头或牙齿。再生需要能够在骨组织中增殖和分化的细胞。虽然有许多可能的人类细胞类型可用作该过程的每个阶段的模型，但没有细胞类型是每个阶段的理想选择。骨肉瘤细胞由于其易于培养和快速增殖而优选用于初始粘附测定，但由于其癌症起源和与正常骨组织的遗传差异，它们不适合用于随后的分化测试。间充质干细胞更适合生物相容性测试，因为它们模拟健康骨中的自然条件，但它们增殖较慢，很快就会衰老，并且某些亚群可能表现出弱的骨分化。原代人成骨细胞在评价生物材料对细胞活性的影响方面提供了相关结果;然而，由于同样的原因，其资源有限，如间充质干细胞。本文综述了骨组织研究中所用材料生物相容性试验的细胞模型。
Bone regeneration after injury or after surgical bone removal due to disease is a serious medical challenge. A variety of materials are being tested to replace a missing bone or tooth. Regeneration requires cells capable of proliferation and differentiation in bone tissue. Although there are many possible human cell types available for use as a model for each phase of this process, no cell type is ideal for each phase. Osteosarcoma cells are preferred for initial adhesion assays due to their easy cultivation and fast proliferation, but they are not suitable for subsequent differentiation testing due to their cancer origin and genetic differences from normal bone tissue. Mesenchymal stem cells are more suitable for biocompatibility testing, because they mimic natural conditions in healthy bone, but they proliferate more slowly, soon undergo senescence, and some subpopulations may exhibit weak osteodifferentiation. Primary human osteoblasts provide relevant results in evaluating the effect of biomaterials on cellular activity; however, their resources are limited for the same reasons, like for mesenchymal stem cells. This review article provides an overview of cell models for biocompatibility testing of materials used in bone tissue research.
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影响因子：
3.1
作者：
Bruderer, M.;Richards, R. G.;Stoddart, M. J.
通讯作者：
Stoddart, M. J.
影响因子：
3.3
作者：
Angelopoulos I;Brizuela C;Khoury M
通讯作者：
Khoury M
雷公藤红素对HOS骨肉瘤细胞凋亡的影响及机制
影响因子：
4.2
作者：
Chen, Yanyang;Ou, Yunsheng;He, Bin
通讯作者：
He, Bin
DOI：
10.1007/164_2019_343
发表时间：
2020-01-01
期刊：
BONE REGULATORS AND OSTEOPOROSIS THERAPY
影响因子：
--
作者：
Ansari, Niloufar;Sims, Natalie A.
通讯作者：
Sims, Natalie A.
DOI：
10.1046/j.1365-2818.1997.1940755.x
发表时间：
1997-04-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
--
作者：
Braet, F;deZanger, R;Wisse, E
通讯作者：
Wisse, E