Characterization of two melanin-concentrating hormone genes in zebrafish reveals evolutionary and physiological links with the mammalian MCH system.
Characterization of two melanin-concentrating hormone genes in zebrafish reveals evolutionary and physiological links with the mammalian MCH system.
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DOI：
10.1002/cne.22171
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发表时间：
2009-12-10
期刊：
影响因子：
--
通讯作者：
Mourrain P
中科院分区：
文献类型：
--
作者：
Berman JR;Skariah G;Maro GS;Mignot E;Mourrain P
黑色素浓缩激素(MCH)调节哺乳动物的摄食和复杂行为，以及鱼类的色素沉着。鱼类和哺乳动物母婴保健系统之间的关系还没有被很好地理解。在这里，我们鉴定和鉴定了斑马鱼中的两个MCH基因，Pmch1和Pmch2。Pmch1及其对应的MCH1多肽与其他鱼类中发现的MCH相似，而斑马鱼Pmch2基因和MCH2多肽与哺乳动物的MCH具有基因组结构、同源性和高度同源性。斑马鱼Pmch基因在下丘脑内紧密联系但不重叠的神经元中表达，MCH2神经元向多个MCH受体丰富的靶点发送大量投射，推测在感觉感知、学习和记忆、唤醒和体内平衡调节中发挥作用。初步的功能分析表明，虽然斑马鱼Pmch1表达的变化与色素变化有关，但MCH2表达的神经元数量随着长期食物剥夺的反应而增加。这些发现表明斑马鱼MCH2可能是哺乳动物MCH的结构和功能同源基因，有助于阐明脊椎动物MCH进化的本质。
Melanin-concentrating hormone (MCH) regulates feeding and complex behaviors in mammals and pigmentation in fish. The relationship between fish and mammalian MCH systems is not well understood. Here, we identify and characterize two MCH genes in zebrafish, Pmch1 and Pmch2. Whereas Pmch1 and its corresponding MCH1 peptide resemble MCH found in other fish, the zebrafish Pmch2 gene and MCH2 peptide share genomic structure, synteny, and high peptide sequence homology with mammalian MCH. Zebrafish Pmch genes are expressed in closely associated but non-overlapping neurons within the hypothalamus, and MCH2 neurons send numerous projections to multiple MCH receptor-rich targets with presumed roles in sensory perception, learning and memory, arousal, and homeostatic regulation. Preliminary functional analysis showed that whereas changes in zebrafish Pmch1 expression correlate with pigmentation changes, the number of MCH2-expressing neurons increases in response to chronic food deprivation. These findings demonstrate that zebrafish MCH2 is the putative structural and functional ortholog of mammalian MCH and help elucidate the nature of MCH evolution among vertebrates.
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影响因子：
7
作者：
Amores, A;Suzuki, T;Postlethwait, JH
通讯作者：
Postlethwait, JH
影响因子：
4.1
作者：
BAKER, B;LEVY, A;LIGHTMAN, S
通讯作者：
LIGHTMAN, S
DOI：
10.1023/a:1014912110318
发表时间：
2001-09-01
期刊：
HISTOCHEMICAL JOURNAL
影响因子：
--
作者：
Duarte, G;Segura-Noguera, MM;Mancera, JM
通讯作者：
Mancera, JM
影响因子：
6.1
作者：
KAWAUCHI, H
通讯作者：
KAWAUCHI, H
影响因子：
4.8
作者：
Kokkotou, EG;Tritos, NA;Maratos-Flier, E
通讯作者：
Maratos-Flier, E