Cherenkov Radiation-Mediated In Situ Excitation of Discrete Luminescent Lanthanide Complexes.
Cherenkov Radiation-Mediated In Situ Excitation of Discrete Luminescent Lanthanide Complexes.
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DOI：
10.1002/anie.201809783
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发表时间：
2018-11-19
期刊：
影响因子：
--
通讯作者：
Boros E
中科院分区：
文献类型：
--
作者：
Cosby AG;Ahn SH;Boros E
镧系元素发光虽然由于光学窗口内尖锐的发射带而非常适合体内应用，但需要高强度、短波长激发镧系元素络合物附近的小型有机“天线”发色团，以通过系间窜越进入激发的 f 轨道状态。在这里，我们探索了放射性同位素 18F 和 89Zr 的切伦科夫辐射作为天线激励的原位源。实现了大环聚氨基羧酸配体的铽(III)络合物的有效分子间和分子内激发(q = 0, phi = 47%)及其功能化附加分子内切伦科夫激发源的类似物(q = 0.07, phi = 63%)。使用传统的小动物荧光成像设备，我们确定在 10 μCi 的 18F 或 89Zr 存在下，检测限为 2.5 nmol [Tb(L1)]- 和 [Tb(L2)]-。我们的系统首次演示了无需外部短波激发的离散发光镧系元素配合物的光学成像。通信激发同位素：由临床使用的放射性同位素发射的切伦科夫辐射被用作生物相容性离散铽(III)络合物的原位、分子间和分子内激发源。证明了使用传统生物成像设备成功检测到发射的镧系元素发光。
Lanthanide luminescence, while ideal for in vivo applications owing to sharp emission bands within the optical window, requires high intensity, short wavelength excitation of small organic “antenna” chromophores in vicinity of the lanthanide complex to access excited f-orbital states via intersystem crossing. Here, we explored Cherenkov radiation of the radioisotopes 18F and 89Zr as an in situ source of antenna excitation. The effective inter- and intramolecular excitation of the terbium(III) complexes of a macrocylic polyaminocarboxylate ligand (q = 0, ϕ = 47%) as well as its analogue functionalized to append an intramolecular Cherenkov excitation source (q = 0.07, ϕ = 63%) was achieved. Using conventional small animal fluorescence imaging equipment, we have determined a detection limit of 2.5 nmol [Tb(L1)]- and [Tb(L2)]- in presence of 10 μCi of 18F or 89Zr. Our system is the first demonstration of the optical imaging of discrete luminescent lanthanide complexes without external short-wave excitation. COMMUNICATION Exciting isotopes: Cherenkov radiation, emitted by clinically utilized radioisotopes, was employed as an in situ, inter- and intramolecular excitation source for biocompatible, discrete terbium(III) complexes. The successful detection of the emitted lanthanide luminescence with conventional bioimaging equipment is demonstrated.
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影响因子：
4.9
作者：
Adams CJ;Wilson JJ;Boros E
通讯作者：
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