Enhancement of Cerenkov luminescence imaging by dual excitation of Er(3+),Yb(3+)-doped rare-earth microparticles.
Enhancement of Cerenkov luminescence imaging by dual excitation of Er(3+),Yb(3+)-doped rare-earth microparticles.
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Er3、Yb3掺杂稀土微粒双激发增强切伦科夫发光成像
DOI：
10.1371/journal.pone.0077926
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Ma X;Kang F;Xu F;Feng A;Zhao Y;Lu T;Yang W;Wang Z;Lin M;Wang J
切伦科夫发光成像（CLI）已成功地应用于临床前研究的各个领域;然而，由于其发光强度弱且穿透能力不足，CLI具有挑战性。在这里，我们报告的设计和合成的一种类型的稀土微粒（REMPs），它可以双重激发的Cerenkov发光（CL）产生的放射性核素的衰变，以提高CLI的强度和穿透。方法：采用水热法制备了Yb 3+和Er 3+共掺杂的六方NaYF 4空心微管。通过X射线粉末衍射（XRD）、扫描电子显微镜（SEM）和分光光度法对样品的物相、形貌和发射光谱进行了表征。一个商业CCD相机配备了一系列的光学过滤器，以量化的强度和光谱的CLI从放射性核素。通过尼龙模体和裸鼠假瘤模型的影像学研究来研究渗透的增强作用。结果：REMPs在520和980 nm波长处可被CL双激发，发射峰在660 nm处重叠。该策略使CLI的总体可检测强度大约增加了一倍，并将其在尼龙模体中的最大穿透力从5 mm扩展到15 mm。在活体动物中的穿透力研究得出了类似的结果。结论：该研究表明，CL可以双重激发REMP，并且在660 nm范围内的重叠发射可以显著增强CL的穿透和强度。所提出的增强CLI策略在未来可能具有很好的应用前景。
Cerenkov luminescence imaging (CLI) has been successfully utilized in various fields of preclinical studies; however, CLI is challenging due to its weak luminescent intensity and insufficient penetration capability. Here, we report the design and synthesis of a type of rare-earth microparticles (REMPs), which can be dually excited by Cerenkov luminescence (CL) resulting from the decay of radionuclides to enhance CLI in terms of intensity and penetration. Methods: Yb3+- and Er3+- codoped hexagonal NaYF4 hollow microtubes were synthesized via a hydrothermal route. The phase, morphology, and emission spectrum were confirmed for these REMPs by power X-ray diffraction (XRD), scanning electron microscopy (SEM), and spectrophotometry, respectively. A commercial CCD camera equipped with a series of optical filters was employed to quantify the intensity and spectrum of CLI from radionuclides. The enhancement of penetration was investigated by imaging studies of nylon phantoms and nude mouse pseudotumor models. Results: the REMPs could be dually excited by CL at the wavelengths of 520 and 980 nm, and the emission peaks overlaid at 660 nm. This strategy approximately doubled the overall detectable intensity of CLI and extended its maximum penetration in nylon phantoms from 5 to 15 mm. The penetration study in living animals yielded similar results. Conclusions: this study demonstrated that CL can dually excite REMPs and that the overlaid emissions in the range of 660 nm could significantly enhance the penetration and intensity of CL. The proposed enhanced CLI strategy may have promising applications in the future.
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