Ionotropic receptors mediate nitrogenous waste avoidance in Drosophila melanogaster.
Ionotropic receptors mediate nitrogenous waste avoidance in Drosophila melanogaster.
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DOI：
10.1038/s42003-021-02799-3
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发表时间：
2021-11-12
影响因子：
5.9
通讯作者：
Lee Y
中科院分区：
文献类型：
--
作者：
Dhakal S;Sang J;Aryal B;Lee Y
氨及其含胺衍生物广泛存在于自然分解副产物中。在这里，我们进行了有偏化学感受器筛选，以研究腐烂水果中不同浓度的铵盐、尿素和腐胺影响取食和产卵行为的机制。我们鉴定了三种离子型受体，包括两种广泛需要的 IR25a 和 IR76b 受体，以及狭义调节的 IR51b 受体。这三种 IR 对于避免含氮废物至关重要，而含氮废物是由苦味味觉受体神经元 (GRN) 介导的。通过表达 Kir2.1 的细胞需求和苦味感受 GRN 中突变体的行为恢复来评估对含氮废物的厌恶。此外，通过进行电生理学测定，我们证实氨化合物具有令人厌恶的味道，因为它们直接激活苦味感知 GRN。因此，我们的研究结果提供了对红外作为检测和避免自然界有毒含氮废物的手段的生态作用的见解。达卡尔等人。对果蝇中的三种离子型受体 IR25a、IR76b、IR51b 使用化学感受器筛选来评估它们对含氮废物回避行为的影响。电生理学检测结果表明，氨化合物通过直接激活苦味味觉受体神经元而产生厌恶味道。
Ammonia and its amine-containing derivatives are widely found in natural decomposition byproducts. Here, we conducted biased chemoreceptor screening to investigate the mechanisms by which different concentrations of ammonium salt, urea, and putrescine in rotten fruits affect feeding and oviposition behavior. We identified three ionotropic receptors, including the two broadly required IR25a and IR76b receptors, as well as the narrowly tuned IR51b receptor. These three IRs were fundamental in eliciting avoidance against nitrogenous waste products, which is mediated by bitter-sensing gustatory receptor neurons (GRNs). The aversion of nitrogenous wastes was evaluated by the cellular requirement by expressing Kir2.1 and behavioral recoveries of the mutants in bitter-sensing GRNs. Furthermore, by conducting electrophysiology assays, we confirmed that ammonia compounds are aversive in taste as they directly activated bitter-sensing GRNs. Therefore, our findings provide insights into the ecological roles of IRs as a means to detect and avoid toxic nitrogenous waste products in nature. Dhakal et al. use chemoreceptor screening on three ionotropic receptors in Drosophila, IR25a, IR76b, IR51b to evaluate their impact on avoidance behaviors against nitrogenous waste products. The results of electrophysiology assays show that ammonia compounds are aversive in taste by directly activating bitter-sensing gustatory receptor neurons.
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影响因子：
16.2
作者：
Koh, Tong-Wey;He, Zhe;Gorur-Shandilya, Srinivas;Menuz, Karen;Larter, Nikki K.;Stewart, Shannon;Carlson, John R.
通讯作者：
Carlson, John R.
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1016/j.tig.2015.09.005
发表时间：
2015-12
期刊：
Trends in genetics : TIG
影响因子：
--
作者：
Joseph RM;Carlson JR
通讯作者：
Carlson JR
影响因子：
8.8
作者：
Chen YD;Dahanukar A
通讯作者：
Dahanukar A
DOI：
10.1073/pnas.0535280100
发表时间：
2003-03-04
影响因子：
11.1
作者：
Gong, WJ;Golic, KG
通讯作者：
Golic, KG