A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi.
A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi.
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DOI：
10.1038/s41598-021-97981-2
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发表时间：
2021-09-16
影响因子：
4.6
通讯作者：
Xie H
中科院分区：
文献类型：
--
作者：
Wang HL;Xu CL;Chen C;Ding SW;Li JY;Yang SH;Xie H
含有LysM结构域的植物蛋白激酶在病原菌识别和自我防御反应中发挥重要作用。它还可以识别微生物相关分子，包括甲壳素和其他多肽。白尖线虫Aphelenchoides Besseyi是一种侵染植物新梢的迁移性寄生线虫。它几乎分布在所有水稻产区，造成高达50%的经济损失。水稻OsRLK3基因是水稻防御相关的LysM激酶基因。本研究表明，flg22、茉莉酸、水杨酸和甲壳素均可诱导水稻LysM激酶OsRLK3的表达。通过酵母双杂交筛选水稻基因OsRLK3与贝氏曲霉cDNA文库的互作，鉴定出一个互作基因AbATPS。AB-ATPS是一个新的ATP合成酶基因，全长1341个核苷酸，编码183个氨基酸。抗-ATPs基因的表达定位于食道和生殖系统。对线虫不同发育阶段的抗体-ATPs的表达进行了评估，发现幼虫的表达最高，其次是卵、雌性和雄性。经抗-ATPS双链核糖核酸(DsRNA)处理的线虫的繁殖力显著降低(p <； 0.05)。瞬时表达实验表明，Ab-ATPS-GFP分布于细胞核、细胞质和细胞膜中，可诱导植物细胞死亡。在接种线虫后的0.5d和1d(p <； 0.0 5)，水稻植株中OsRLK3的表达显著增加。此外，在0.5d时，抗-ATPS双链RNA处理组的OsRLK3表达显著低于GFP双链RNA处理组(p <； 0.05)，在第1天显著高于GFP双链RNA处理组(p <； 0.05)。这些结果表明，水稻OsRLK3可以与黄曲霉抗体-ATPS相互作用，这在该线虫的生长、繁殖和侵染过程中起着重要作用。我们的研究结果为进一步了解白纹伊蚊的寄生策略及其与寄主植物的相互作用机制提供了理论依据，为防治白纹伊蚊提供了新的思路和靶标。
Plant kinases containing the LysM domain play important roles in pathogen recognition and self-defense reactions. And it could recognize microbe-associated molecules including chitin and other polypeptides. The white tip nematode Aphelenchoides besseyi is a migratory parasitic nematode that infects plant shoots. It is distributed over almost all rice-producing areas and causes up to 50% economic losses. The rice OsRLK3 gene was a defense-related LysM kinase gene of rice. This study showed that the rice LysM kinase OsRLK3 could be induced by flg22, jasmonic acid, salicylic acid, and chitin. An interaction gene, Ab-atps from A. besseyi, was identified by screening the interaction between the rice gene OsRLK3 and an A. besseyi cDNA library using yeast two-hybrid screening. Ab-atps is a novel ATP synthase gene with a full length of 1341 bp, coding for 183 amino acids. The mRNA of Ab-atps was located in the esophagus and reproductive system of A. besseyi. The expression of Ab-atps was assessed at different developmental stages of the nematode and found to be the highest in the juvenile, followed by the egg, female, and male. Reproduction was significantly decreased in nematodes treated with Ab-atps double-stranded RNA (dsRNA) (p < 0.05). Transient expression experiments showed that Ab-ATPS-GFP was distributed in the nucleus, cytoplasm, and cell membrane, and Ab-ATPS-GFP triggered plant cell death. OsRLK3 was expressed significantly higher at 0.5 day and 1 day (p < 0.05) in rice plants inoculated with nematodes treated with Ab-atps dsRNA and gfp dsRNA for 0.5–7 days, respectively. Further, OsRLK3 expression under Ab-atps dsRNA treatment was significantly lower than with gfp dsRNA treatment at 0.5 day (p < 0.05) and significantly higher than with gfp dsRNA treatment at 1 day (p < 0.05). These results suggest that rice OsRLK3 could interact with A. besseyi Ab-atps, which plays an important role in growth, reproduction, and infection of the nematode. Our findings provide a theoretical basis to further understand the parasitic strategy of A. besseyi and its interaction mechanism with host plants, suggesting new ideas and targets for controlling A. besseyi.
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影响因子：
5.6
作者：
Newman MA;Sundelin T;Nielsen JT;Erbs G
通讯作者：
Erbs G
影响因子：
5.5
作者：
Allman, Erik;Johnson, David;Nehrke, Keith
通讯作者：
Nehrke, Keith
影响因子：
11.6
作者：
Balhadère, PV;Talbot, NJ
通讯作者：
Talbot, NJ
影响因子：
1.5
作者：
Chaves, I.;Alves, M.;Regalado, A. P.
通讯作者：
Regalado, A. P.
DOI：
10.1073/pnas.0705147104
发表时间：
2007-12-04
影响因子：
11.1
作者：
Miya, Ayako;Albert, Premkumar;Shibuya, Naoto
通讯作者：
Shibuya, Naoto