Dynamic hydraulic flow stimulation on mitigation of trabecular bone loss in a rat functional disuse model.
Dynamic hydraulic flow stimulation on mitigation of trabecular bone loss in a rat functional disuse model.
复制标题
DOI：
10.1016/j.bone.2012.06.030
复制
发表时间：
2012-10
期刊：
影响因子：
4.1
通讯作者：
Qin, Yi-Xian
中科院分区：
文献类型：
--
作者：
Hu, Minyi;Cheng, Jiqi;Qin, Yi-Xian
关键词：
骨液流动（BFF）已被证明是机械传导信号和骨适应的关键调节因子。髓内压（ImP）和基质应变被认为是调节BFF的潜在发生器。为了使用ImP提高体内振荡BFF，开发了动态液压刺激（DHS）方法。本研究的目的是评估DHS对减轻大鼠后肢悬吊（HLS）功能废用模型中骨丢失和结构改变的影响。将61只5月龄雌性Sprague-Dawley大鼠分为5组：1）基线对照组，2）年龄匹配对照组，3）HLS组，4）HLS +静态负荷组，5）HLS + DHS组。每天在胫骨区域以“10分钟开-5分钟关-10分钟开”的负荷方案进行液压流动刺激，每周5天，总共4周。使用µCT和组织形态计量学分析近端胫骨的干骺端小梁区域。四周的HLS导致骨小梁的显著损失，导致结构恶化。单纯静态负荷的HLS不足以减轻骨丢失。与HLS对照组相比，DHS负荷显著改善了骨量和微结构，导致骨体积分数增加83%，骨小梁数量增加25%，骨小梁分离减轻约26%。骨小梁矿化的组织形态计量学分析与µCT分析一致，其中DHS负荷导致组织形态计量学BV/TV增加34%，MS/BS增加121%，BFR/BS增加190%，BFR/BV增加146%。总体而言，数据表明，动态液压流量负荷有可能提供调节信号，以减轻功能废用引起的骨丢失。这种方法可能为未来骨质疏松症的临床治疗提供一种新的替代机械干预方法。
Bone fluid flow (BFF) has been demonstrated as a critical regulator in mechanotransductive signaling and bone adaptation. Intramedullary pressure (ImP) and matrix strain have been identified as potential generator to regulate BFF. To elevate in vivo oscillatory BFF using ImP, a dynamic hydraulic stimulation (DHS) approach was developed. The objective of this study was to evaluate the effects of DHS on mitigation of bone loss and structural alteration in a rat hindlimb suspension (HLS) functional disuse model. Sixty-one 5-month old female Sprague-Dawley rats were divided into five groups: 1) baseline control, 2) age-matched control, 3) HLS, 4) HLS + static loading, and 5) HLS + DHS. Hydraulic flow stimulation was carried out daily on a “10 min on-5min off-10min on” loading regime, 5 days/week, for total of 4 weeks in the tibial region. The metaphyseal trabecular regions of the proximal tibiae were analyzed using µCT and histomorphometry. Four weeks of HLS resulted in a significant loss of trabecular bone, leading to structural deterioration. HLS with static loading alone was not sufficient to attenuate the bone loss. Bone quantity and microarchitecture were significantly improved by applying DHS loading, resulting increase of 83% in bone volume fraction, 25% in trabecular number and mitigation of -26% in trabecular separation compared to HLS control. Histomorphometry analysis on trabecular mineralization coincided with the µCT analysis, in which DHS loading yielded increases of 34% in histomorphometric BV/TV, 121% in MS/BS, 190% in BFR/BS and 146% in BFR/BV, compared to the HLS control. Overall, the data demonstrated that dynamic hydraulic flow loading has potentials to provide regulatory signals for mitigating bone loss induced by functional disuse. This approach may provide a new alternative mechanical intervention for future clinical treatment for osteoporosis.
登录
查看更多内容
影响因子：
--
作者：
JEE, WSS;WRONSKI, TJ;KIMMEL, DB
通讯作者：
KIMMEL, DB
DOI：
10.1073/pnas.91.9.3569
发表时间：
1994-04-26
影响因子：
11.1
作者：
KASTEN, TP;COLLINOSDOBY, P;NICKOLS, GA
通讯作者：
NICKOLS, GA
影响因子：
1.9
作者：
Lau RY;Guo X
通讯作者：
Guo X
影响因子：
4.1
作者：
Kameyama, Y;Hagino, H;Teshima, R
通讯作者：
Teshima, R
影响因子：
6.2
作者：
Hsieh, YF;Turner, CH
通讯作者：
Turner, CH